9:30 am – 9:15 pm
- working on / revising BW proposal
- two more rounds of comments from Carl
- comments from Jeff.
Serious problems introduced by microscope A
- recent bead data sets collected on new version of software are NOT renormalized to maintain full dynamic range and constant contrast! Need to fix this in
- Dave destroys my conv_bk backup movies, does crazy unexpected stuff with power files and laser levels (blasts my sample with UV). This unanticipated behavior is highly destructive to my sample and data quality.
- Unexpected behavior in dave is due to the entirely different way dave parses shutter files. This needs to be fixed before imaging more.
- Running chromatic bead analysis. Quad view horribly misaligned — require match radius of greater than 30 pixels to match beads.
- interesting article on transcription vs translation as determinant of protein levels. Also a good example of reusing public data sets.
(Good article by Thomas, Hernan and Shawn as well).
5:30 pm – 12:10 am
- Analyzing recent multi-color chromatin data with
- not dramatically different overlap/exclusion between yellow-yellow and yellow-black
- yellow-black is however a ‘closer’ boundary (at least in the black-to-yellow direction) since the black domain is more compact
- should also do some modeling on this to better inform intuition.
Some snapshots from today’s analysis
- Interesting review article by Pirrotta on new papers arguing PRC1 then PRC2
Maintaining the living things
- passage cells
- flip fly stocks
11:00 am – 5:00 pm, 8:00 pm – 11:00 pm.
- started work on BW fellowship app
- setup account, started filling in general information
- started new folder in
- revising STORM uncertainties section
- clarified discussion of geometric distributions
- discussed Xist PRC2 SIM paper
- Cajal teminated remote desktop.
- messed around with activating licenses, got it to recognize its licenses finally. Did not need to re-enter the keys.
- analyzed E8 E9 data.
- First 10 movies look pretty decent.
- There-after the buffer is pretty dead, 750 data quality drops through the floor.
- I wish these MEA-COT buffers would live longer like the BME-COT buffers. No dice.