About my notebook

This is my lab notebook.  It is how I organize and document my scientific research on a day-to-day basis.  It is intended primarily for my personal use as a permenant record of my work.

Unlike traditional notebooks, it is text searchable.  All notes are listed in appropriate categories, and I can easily rearrange my notebook to display only posts relating to a particular category.  Research images, from gel photos to simulation results to draft figures for manuscripts are included as images, and automatically become part of a date-tagged browsable image collection.  Hyperlinks let me connect protocols and references.

My notebook also automatically tracks my code development, through RSS feed forwarded by my code repository in Github.  The notebook additionally keeps track of what I’m reading, through RSS feed run through Mendeley (when the API works).

In addition to being more convenient, I hope this notebook contributes in a small way to making Science more Open.  Much of what we do as academic researchers is never published, and these findings (though funded generally by tax payer dollars) are forever lost to the world.  Much of what is published is only available in expensive, specialized journals which most of the world does not have access to.  It is my hope, and that of the Open Science Movement, that the technologies of Web2.0 enable us to move to a world where this is no longer the case.  In an open or partially open web hosted notebook, all the unpublished ideas and discoveries can still be easily released to the world, where they are text-searchable and easily discoverable, so that other researchers can benefit from them.

Unpublished information that I am not yet ready to share with the world at large is protected by passwords.  These can be shared with individual collaborators of mine, and removed once the relevant work is published.

I have received much inspiration, encouragement, and technical support from several individuals.  Most importantly, my brother Carl Boettiger, who is a leading figure in the Open Science Movement at Davis, and keeps a Open Notebook here.

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Protected: Friday 02/26/16

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Protected: Alex Pollen — human vs chimp brain organization

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Thursday, 2/25/16

Seminars

  • Alex Pollen seminar (see notes)

Microscope building

  • HHMI equipment cycle equipment granted was 3 MPB lasers (560, 647, 750), Hamamatsu camera, Nikon microscope body w/ 8K of extras, Coherent 488 laser
  • submitted separate quote addressed to Harvard for
    • optics table + legs
    • 405 laser
  • need quotes for
    • AOTF
    • quadview
    • piezo
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Protected: Monday 02/22/16

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Friday 02/19/16

9:00 am – 5:00 pm

Tasks

  • lab meeting 10 am – 12:30 pm (see notes)
  • FISH with Hazen — prepped cells up to hyb step, should be in formamide by tonight
  • Reply to request from Biteen lab for code help
  • Phone interview with UCSD Epigenomics and chromatin, joint search
  • Receipt for HMS visit
  • recommend authors/papers/subjects for Ann Rev Biophys for XZ (see email)
  • got schedule for cornell visit

To do still

  • schedule caltech flights
  • NE Dev Bio application and abstract (?)
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Protected: lab meeting 02/19/16

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Protected: thoughts on improving my chalk talk

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Thursday 09/18/16

9:30 am – 9:00 pm

Tasks

  • reply to MIT IMES / Physics
  • reply to Caltech
  • submit patent signatures
  • Discussion with Hsuan – microscopes and plans
  • Discussion with BB
    • using common label as a fiducial for XYZ corrections
    • validate if I have a Cy3 common for STORM activation on L7 – yes arrived today.
    • parts not all here for flow system: waiting for needles and power-supplies (Hamilton not stock power supply, ordered from Amazon).
    • need more scope time for experiments.
    • test brightness on current data

Still to do

  • NEBSB abstract?

MERFISH code

  • final debugging of library building code in test mode.
  • annotating code
  • push back to repo
  • test JM’s code scripts
    • error on read info file — bin files found but inf files not bound
    • line 33 of analysis_script calls parameters.codebook, but parameters.codebook is not defined.
    • Changing this to parameters.codebookPath (defined on the previous line), lets the CodebookToMap function run.

Scope building

New quotes

  • AOTF
  • Quadview
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Monday 09/15/16

10:07 am –

Tasks

  • Discussion about super-resolution of individual membrane proteins (EL lab).
  • Reply to request for data from JM Victor.
  • Project updates with BB. Need to contact LM team about explaining model.
  • Discussion on quantifying nuclear structures with new postdoc (see email). Promised to send matlab code.
  • Reviewing research interests and publications from HMS prior to this week’s meetings.
  • Sent abstract to Cornell for discussion
    • minor updates to slides
    • reading Schrodinger’s What Is Life (a useful context for this talk I think).
    • introduction still needs substantial work.
  • Working on public domain code for MERFISH
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