Tag Archives: writing

Thursday 11/15/12

Posted on November 15, 2012 by admin

9:15 A — 6:45 P, 8:30P – 12:00A collect virgins for sim Espl[D] recombineering new cross, sna males x Sp/CyoZ; TM2/TM6 virgins clear MTDs and TM2/TM6s for virgin collection (peak emergence for latest bottles?) pulverize and refreeze Espl[D] and sim[D] … Continue reading →

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Wednesday 11/14/12

Posted on November 14, 2012 by admin

9:00A – 7:30P, 8:15P – 11:15P Prepping gel (low on Na Borax, and TAE making up new TAE) Test gel: YW postive control AbdB looks great.  YW negative control sim[D] is clean.  Improvised gel extractions not looking good. Try sim[D] … Continue reading →

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Sunday 11/11/12

Posted on November 11, 2012 by admin

10:15 A – 5:00 P, 8:20 – 9:00P working on BJ review figures write BJ review captions review NSF proposal Start Analysis of Inv data  Run bead analysis Need to fix crazy quadview offset from STORM 4 with a translation … Continue reading →

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Thursday 11/07/12

Posted on November 8, 2012 by admin

9:30 A -8:00P, 9:30-11:00P check STORM.  — run finished without losing focus. Summary of inv run:  Unfortunately 514 doesn’t work either as a double label. inv-Epc male a lot of promising double spots, but it’s a litter harder to sort … Continue reading →

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Sunday 11/04/12

Posted on November 4, 2012 by admin

11:00 A – 8:00P Cell culture and cell labeling Start washing out direct-labeled primaries from cell-culture plates. Split cells, toss old plates. plate cells on round cover glass in 12 well pates.  See if these adhere any better for FISH-on … Continue reading →

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Saturday 11/03/12

Posted on November 3, 2012 by admin

10:45 A – Writing Write BJ review Drosophila PWMs TF binding motifs FlyFactor Ecoli TF binding + PWMs here (might be something better) Finish first draft through of enhancer integration section.  On to promoters next. DFISH on cell culture remove … Continue reading →

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Wednesday 10/31/12

Posted on October 31, 2012 by admin

9:45 A – 8:40P, 9:45P-11:45P Submit revised snail kinetics paper. Collect virgin Espl/sim cross to TM2/TM6 Collect virgin Act5 Gal4.  Cleared and added new bottle of these to virign collection. washout Pc antibody from O/N MTD post fix 5 min … Continue reading →

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Friday 10/26/12

Posted on October 26, 2012 by admin

10:20 A – 7:30PM Fly Work  Set up collection cages for TRiP knockdown experiments: MTD x Psc 1 MTD x Pc 3 Set up new collection cage of MTD controls.  Pc labels looked weak and Pc in situs did not … Continue reading →

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Thursday 10/25/12

Posted on October 25, 2012 by admin

9:50 A – 7:00P, 8:30P – 11:20 P Clean and coat coverslips washout fix from O/N fix samples, move to EtOH rehydrate, check for presence of staining. Respond to team about NSF BIRS proposal Pol I arrived 4:00 PM check … Continue reading →

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Wednesday 10/24/12

Posted on October 24, 2012 by admin

9:15 A – 7:00P, 8:00P – 12:00A RNA FISH + Immuno-stain in cultured cells (developing protocol:) warming up hybe solution for hot washes remove, label and save probes (in yellow -20C dilute probes box, labeled as S2). 3x Hot wash … Continue reading →

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