Tag Archives: literature

Tuesday 10/09/12

Posted on October 9, 2012 by admin

10:00 A – 7:20P, 8:30P – 11:30P Fly Work collect virgin MTDs.  (progeny of MTD x TRIP -> collection cage.   These embryos should lay infertile offspring / transformed embryos).  Let’s do Psc; MTD first, see if structure of K27me3 … Continue reading →

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Friday 08/31/12

Posted on August 31, 2012 by admin

9:30 A – 7:30P, 10:30 – 12:50A Break down O/N mp10a run. save mp07 data.  All look good, 7 images finished completely, last image starts at the top of the mRNA field. etching S1 AbdA, AbdB, Ubx, K27me3. Issues booting … Continue reading →

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Reflections on Petruk et al Cell 2012

Posted on August 26, 2012 by admin

TrxG and PcG Proteins but Not Methylated Histones Remain Associated with DNA through Replication, Cell (2012), Result 1 (Fig 1):  ‘K27’ ‘K4’ don’t associate with PCNA labeled nuclei.   comment: At gastrulation K27 and K4 levels are much much lower than … Continue reading →

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Monday 05/28/12

Posted on May 28, 2012 by admin

10:40 A – 7:00P, 8:45P – 11:45 P Flip fly bottles: Pc-GFP.  Now growing at 25C Flip into single bottles for bottle stock: Pc-shRNA1 and 2, Psc-shRNA1,  Bcd-GFP, eve-GFP, en-GFP.  Pc-shRNA lines strongly female gender biased. Primary stocks at 18C look … Continue reading →

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Tuesday 07/05/11

Posted on July 5, 2011 by admin

9:50 A — 7:00 P, 7:40 P – 8:15P Research planning from lab meeting discussion. Literature browsing, nothing striking. Need repeat lab meeting on Friday Collating snail data in preliminary figures Order new flies: Dp(1;Y)BSC172, y[+] P{w[+mW.Scer\FRT.hs3]=3′.RS5+3.3′}BSC9, B[S]/winscy (brk duplication … Continue reading →

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Wednesday 06/29/11

Posted on June 29, 2011 by admin

9:40 A –3:30 P, 7:50 P – 9:20 P Finish ON confocal MP07Hz_snaD image 2.8Hz_snaD rescue for counting.  — still drift issue during acquisition as lens heats. Literature review: Set1 not Trx is major H3K4 trimethyltransferase (Lis) Trans-activation in mammalian … Continue reading →

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