Tag Archives: presentation

Thursday 05/10/12

Posted on May 10, 2012 by admin

10:00A -8:00P working on chromatic correction for beads to apply to molecule lists Finished script to merge multiple bead fields from confocal and compute transforms. working on intro sections of ESF talk, formatted to work on laptop. comments back from … Continue reading →

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Wednesday 05/09/12

Posted on May 9, 2012 by admin

12:30 P – 9:45 (volleyball practice 8:30A-11:30A) Pellet and resuspend all new probes. updated probe & plasmid database with new & resynthesized probes: [spreadsheet 0AjSqkxgziU1YdHlnZmFzOVBsX0JQUjlrQnRUWjVYVGc 600 400 sheet=1] 3x maternal Gal4 flies arrived from Perrimon lab -> start expanding coding molecule … Continue reading →

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Monday 05/07/12

Posted on May 7, 2012 by admin

9:50A – 8:30 P Sang-Hee lab meeting Sonny JC (Ringrose paper and Psc as cell cycle regulator paper) laying out presentation for ESF conference

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Monday 04/30/12

Posted on April 30, 2012 by admin

9:45 A – 8:15P, Steven group meeting presentation sequencing results: AbdB-1A: sense strand.  Use SP6 for antisense probe AbdB-1B: sense strand.  Use SP6 AbdB-3A: sense strand. poor quality read AbdB-3B: antisense strand. Use T7 Dfd-2A: antisense strand: T7 Dfd-2B: antisense … Continue reading →

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Monday 04/23/12

Posted on April 23, 2012 by admin

9:45 A – 11:30P Lab meeting presentation John Wu’s thesis defense Consolidated disk drives: new data on external drives Alistair4 and Alistair5 backed up to empty gaps slots on Alistair2 and 3. Flip fly cages – 2:30P Analyze Psc localization, … Continue reading →

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Sunday 04/22/12

Posted on April 22, 2012 by admin

10:30 A -5:00P Fly work Flipped all Gal4-Bcd3’UTR lines into new bottles (8).  Moved to 25C.  Flip again in 2-3 days, start virgin collection in 10 days. New cages of MP09, yw, and Pc-GFP. Disposed all bottles of MP09 and … Continue reading →

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Saturday 04/21/12

Posted on April 21, 2012 by admin

10:45 A – 7:45 P, 10:30P – 11:30 P Working on lab meeting presentation slide organization new analysis of Psc, H3K27, H3 localization differences HP1a doesn’t haven’t correct conventional level nuclear localization Samples to cut and image 4H  — K27me3-750, … Continue reading →

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Friday 03/20/12

Posted on April 20, 2012 by admin

11:55 A – 8:00P, 9:30 – 11:30P (volleyball 8:50A-11:50A) need missing reference letter and electronic submission for ASC app! running STORM analysis on 04/05/12 En-tou-Epc-H3 data. Rerunning chromatic alignment on 04/14/12 en-Epc conventional data working on slides for group-meeting presentation

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Thursday 04/19/12

Posted on April 19, 2012 by admin

9:50 A – 8:00P, 9:30 – 10:30P Working on group meeting presentation background slides figure out and implement gaussian dot fitting in matlab use precise fitting to locate molecules in conventional images of En-Epc-H3 stained embryos, superimpose chromatically corrected images. … Continue reading →

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Wednesday 04/11/12

Posted on April 11, 2012 by admin

10:00A – 11:30P coating 5o mm coverslips put coverslips in metal, vertical mount holder.  Don’t stay balanced in ceramic holder.  Need to alternate positions or the coverslips stick. Do coating in 400 mL in 1000 mL beaker (not 300 mL … Continue reading →

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