Tag Archives: writing

Saturday 03/30/13

11:15 A – 8:15 P, 9:30 P-11:55 P Review working on explanation of individual site contributions to cooperativity: Jiang 1993 Lebrecht et al 2005 make Bcd rescue embryos lacking the Bcd interaction domain required for cooperativity in vitro. They observe … Continue reading

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Friday 03/29/13

9:30 A – 8:10 P, 9:00 P – 12:10 A Install cryo-attachment be sure to retract arm and center FC mount prior to mounting be sure there is space between the arm and the chamber so the arm can move … Continue reading

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Wednesday 03/27/13

10:00 A – 7:00 P, 9:30 P – 12:20 A STORM Break down O/N STORM run on STORM3 of fab7-405-cy5 STORM2 has issues Iris for columnating optics is too small for 512×512 view Focus lock can’t get minimal signal TIRF … Continue reading

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Tuesday 03/26/13

10:30 A – 7:30 P, 8:30 P – 10:30 P Tx-review working on literature references, laying out introduction. Stinchcombe model write out probability mass function of discrete mRNA arrivals in terms of the random differential equation for the continuous mRNA … Continue reading

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Sunday 03/10/13

9:30 A – 12:15 A Meeting with Ajaz oops it’s daylight savings day! Ajaz will induce and fix cells, bring over fixed cells on coverglass label with m-anti-Flag + A647-A405-dk a-m secondary and STORM. Compare clusters. STORM Finished O/N imaging … Continue reading

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Saturday 03/09/13

10:30 A STORM setup imaging of 2x-Cy5 labeled Fab7. Hardly detectable loci in conventional — many (most?) cells no candidate locus detectable. General cy5 background seems higher. Found a few cells that look like reasonable candidates for conventional. Background clearly … Continue reading

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Friday 03/08/13

10 A – 11:00 P Transcription models Finished derivation from yesterday on multiple binding wrote up in Review, started extension to cooperativity Markov Chains in TeX write like chemical equations with mhchem package Use Tikz drawing package Named Graphs (Tikz … Continue reading

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Thursday 03/07/2013

9:30 A – 11:10 P Coding Reorganizing Image_Analysis Repository New folder for 2013 Cell Reports paper Major rewrite of LSM parsing code And 6 hours later: LSMextract.m – Takes a .lsm file, extracts all z-positions and all stage-positions into multicolor … Continue reading

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Wednesday 03/06/13

9:50 A- 6:00P, 9:00P-10:00P (remotely) General receive and freeze Jeff’s CustomArray order configure Windows Backup to backup onto Drive D. Contact co-organizers about setting up Speaker’s List, Talk Titles, Activities Page, Transport Page. Embryo Work Flip Esc collection cage, ~11A … Continue reading

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Sunday 12/23/12

Working on bistability paper

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