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Thursday 04/25/13
10:30A – 6:30P, 8:45 P – 11:30 P Working on Presentation for Chicago added slides on changing number of dorsal sites in snail enhancers as an example of site redundancy could use the UAS-site multerimerization tested at Jannelia as synergistic … Continue reading
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Protected: project 2 to do
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Lander seminar
Background * PhD in Math from Oxford, undergrad at Princeton, started as a professor in business school at 24 * Met David Botstein to integrate in genetics and mathematics * Sydney Brenner on “omics” has created the idea that if … Continue reading
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Wednesday 04/24/23
10:00A – 7:45P, 8:30P – 11:15P Project 2 notes OligoPaints More troubleshooting diagnostics Gel order #2 final, #3 final, y10mL DE, B10ml DE, #6 PCR, # 7 PCR, #9 PCR, #8 PCR, #4 EP1, #5 EP1, y-assym column, b-assym column, … Continue reading
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Abstract for Chicago talk
Title: “Elucidating principles of gene regulation from stochastic models” Abstract: The complexity of multicellular organisms arises largely from reusing many of the same genes in numerous combinations, rather than by the introduction of novel genes for each new cell-type. Put … Continue reading
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Tuesday 04/23/13
9:15 A – 12:15 A STORM Finish STORM O/N run OligoPaint probe synthesis Ubx on 50C denaturing gel looks decent. nice tight band large band after PCR, splits into two bands after nicking. Unfortunately most of the product is not … Continue reading
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Monday 04/22/13
9:00 A – 7:00 P, 8:40 P – 12:20 A check concentrations Measured in ug total DNA Sample PCR ng/uL Filtration EtOH EtOH no glyc Nicking #1 Y nick 41.8 N/A 36 37 10 #2 B nick 26.4 N/A 21 … Continue reading
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