Author Archives: admin

Protected: lab meeting 04/06/13

Posted on April 8, 2013 by admin

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Finite state Markov model notes

Posted on April 6, 2013 by admin

simple switching model. Two systems, 1 and 2, each two states A and B. fi Ai <==> Bi bi Bi –> Bi + mRNA A1 <==> A2 [* f1 s12 0 b1 * 0 0 s2 0 * f2 0 … Continue reading →

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Friday 04/05/13

Posted on April 5, 2013 by admin

9:00 A – 8:00 P, 9:00 P – 10:45 P General (8:00 A allergy test) STORM analysis Getting images of relative background levels in 3-Cy5 vs 405-cy5 Drift correction makes sparse images look horrible (even the rather long integration of … Continue reading →

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Protected: Activator oligoPaints: results summary 04/05/13

Posted on April 5, 2013 by admin

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Protected: issues with ph-FLAG round 1

Posted on April 5, 2013 by admin

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oligopaint-PCR

Posted on April 4, 2013 by admin

Calculating concentrations (Qubit) and molecule numbers: starting primer = 30 ng in 300 uL in 1 ng/uL, added 100 uL = 100 ng 100 uM = 100E-12 mols/uL add 100 uL, = 10 nmols starting primer. Y PCR: 73.7 ng/mL … Continue reading →

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Protected: STORM3 fab7 data

Posted on April 4, 2013 by admin

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Thursday 04/04/13

Posted on April 4, 2013 by admin

9:30 A STORM Finish O/N STORM run of 3x Cy5 512×512 on STORM2 # Figure out were to transfer data Modeling # Reply to Chris STORM Analysis Implement automatically detected, variable size window to STORMrender. A lot of Ph movies … Continue reading →

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Wednesday 04/03/13

Posted on April 3, 2013 by admin

9:45 A – 8:00 P, 9:30 P – 12:00 A General Send notes on sog data to Mounia Training on Qbit. Important point: measure samples between 2 and 2.5 minutes after mixing in reaction dye. STORM Analysis Manual analysis of … Continue reading →

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Tuesday 04/02/13

Posted on April 2, 2013 by admin

9:45A – 5:00P, 8:00P – 10:00P Modeling Exploring parameter space of site-interaction-number models. Bursty regime: fraction of time in the transcribing state is low (kon/(kon+koff)) Transcription rate in the on phase cooperativity noise Most obvious at low induction In general … Continue reading →

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