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Tuesday 03/26/13
10:30 A – 7:30 P, 8:30 P – 10:30 P Tx-review working on literature references, laying out introduction. Stinchcombe model write out probability mass function of discrete mRNA arrivals in terms of the random differential equation for the continuous mRNA … Continue reading
Monday 03/25/13
9:50 A – 7:45 P, 9:30 P – 12:15 A Lab Meeting (notes) Journal Club: (notes) Research update meeting preparing update on multi-color DFISH No data on BX-C vs Flank, I recall it didn’t work well but I thought I … Continue reading
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Protected: preliminary BX-C embryo
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Journal club: Aberrant light directly impairs mood
Aberrant light directly impairs mood and learning through melanopsin-expressing neurons LeGates et al 2012 (presented by colenso) Circadian rhythm Natural pacemaker controls circadian rhythm. 50% of blind individuals experience ‘free running’ – no entrainment to light. about a 25 hr … Continue reading
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Protected: lab meeting 03/25/13
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Posted in Lab Meeting
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Sunday 03/24/13
11:00 A – 7:40 P Transcription modeling Failed to derive general case transcription rate for arbitrary number of transcriptionally competent states: Failed to derive probability density function for steady state distribution of mRNA transcripts (allowing for decay). Referee Complete, proofread … Continue reading
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Saturday 03/23/13
11:00 A Cell culture Moved cell to 28C incubator observe substantial cell debris in dish (dead cells from heat shock?) Genomics Installing OligoArray2.0 http://berry.engin.umich.edu/oligoarray2/ Need to install legacy BLAST. Installed. Need to install OligoArrayAux: http://mfold.rna.albany.edu/?q=DINAMelt/OligoArrayAux. Error with database in2 = … Continue reading
Friday 03/22/13
11:00 A – 7:30 P, 9:30P – 11:58 P Discussion recommend using homology-fused single labeled probes to test the double labeling efficiency for Hao’s experiment. reference call for Bryon Genomics cleaned up Genomics functions maxInterval.m maxIntervalBed.m added new function IntervalFuseGaps.m … Continue reading
Saturday 03/16/13
11:00 A – 7:00P Kinetic modeling STORM Analysis Switching is indeed failing on BX-C 2/20 data. Sad we still don’t have this basic data with enough depth yet. S3 cell data shows plenty of blinking out at 58 and 90K … Continue reading
Posted in Summaries
Tagged coding, images, modeling, STORM analysis
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Friday 03/15/13
9:50 A – 8:30 P Goals Write SDB abstract. OligoPaint prep * Run probe design * Order probes 3. Chromatin color region selection * write script to chose genomic regions based on size, color, and color-confidence score. Genomics Probe Design … Continue reading