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Author Archives: admin
Saturday 03/09/13
10:30 A STORM setup imaging of 2x-Cy5 labeled Fab7. Hardly detectable loci in conventional — many (most?) cells no candidate locus detectable. General cy5 background seems higher. Found a few cells that look like reasonable candidates for conventional. Background clearly … Continue reading
Friday 03/08/13
10 A – 11:00 P Transcription models Finished derivation from yesterday on multiple binding wrote up in Review, started extension to cooperativity Markov Chains in TeX write like chemical equations with mhchem package Use Tikz drawing package Named Graphs (Tikz … Continue reading
Code updates
updating other changes in directory. Old files may get restructured here soon into subdirectories. + .gitignore + Perry2011PNAS/read_planeT.m m Unsupervised_DotFinding.m + imclean.fig m imnucseg_pars0.mat m imnucseg_pars1.mat + imviewer.fig + imviewer_lsm.fig m imviewer_lsm.m m jacquestiffread.m updating other changes in directory. Old … Continue reading
Posted in Microscopy
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Thursday 03/07/2013
9:30 A – 11:10 P Coding Reorganizing Image_Analysis Repository New folder for 2013 Cell Reports paper Major rewrite of LSM parsing code And 6 hours later: LSMextract.m – Takes a .lsm file, extracts all z-positions and all stage-positions into multicolor … Continue reading
Wednesday 03/06/13
9:50 A- 6:00P, 9:00P-10:00P (remotely) General receive and freeze Jeff’s CustomArray order configure Windows Backup to backup onto Drive D. Contact co-organizers about setting up Speaker’s List, Talk Titles, Activities Page, Transport Page. Embryo Work Flip Esc collection cage, ~11A … Continue reading
Posted in Summaries
Tagged embryo collection, presentation, writing
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Tuesday 03/05/13
9:50 A – 7:30 P, 8:30 P – 10:50 P General Discussing replies for Banff invites. Student substitutions add to bottom of list Still need to write more acknowledgement of replies. STORM Analysis launch analysis of all unprocessed datafiles. ESATA … Continue reading
Posted in Summaries
Tagged coding, images, open problem, section staining, troubleshooting
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embryo sections
some sections lie nice and flat, others get substantial ripples. Is this knife compression? too large a section in x-y? (seems to correlate). Too thick a section? (seems also to be true in my experience).
Monday 03/04/13
9:45 A – 7:30 P, 9:00 P-11:30 P General stuff Group meeting: see notes send out invites to Banff workshop Continue working on writing referee review Fly work flipped all bottle stocks tossed all Mat alpha tub bottles currently expanding: … Continue reading
Michelangelo D’Agostino: Physics and presidential politics
Background harvard undergrad, berkeley PhD, worked on Icecube (neutrino detection) Approach neural networks and support vector machines to score liklihood of particular outcomes (neutrinos or voter stats) Data collection — mass profile data from Facebook / Twitter. Campaign organization Tech … Continue reading
Posted in Seminars
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Journal club: Lee .. Ha, Science 2013
Jeff Moffitt Elastic coupling between RNA degradation and Unwinding by Exoribronuclease (Lee … Ha, Science 2013) Exosome at core of RNA modification / degradation in yeast. complex conserved through domains of life. called PNPase in bacteria some core elements of … Continue reading
Posted in Journal Club
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