Author Archives: admin

Tuesday 06/03/14

Posted on June 3, 2014 by admin

9:30a – 3:45p, 7:00p – 9:00p schedule 11:00a-12:00p, volleball match 1:45-3:00p doctor’s appt 3:45p-7:00p feeling sick gone home Chromatin STORM imaging B09 Data analysis Analyzing small region yellow data: B03 and B04

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Monday 06/02/14

Posted on June 2, 2014 by admin

9:45am – 4:45pm, Goals Set up RT reactions Lib3 samples B10-B12, C09-C12, D01-D08 Wash newly stained samples (B08, B09, E11-F01, + PH immuno-FISH) set up STORM imaging of PH immuno-FISH Lab Meeting see notes General letterhead and signature for letter … Continue reading →

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Protected: Jiang lab meeting 05/02/14

Posted on June 2, 2014 by admin

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Sunday 06/01/14

Posted on June 1, 2014 by admin

10:00am – 7:00pm Goals Call Otto’s order pizza for lab meeting (done) Finish lcPCR and cleanup (done) Set up T7 reactions (done) post-fix Ajaz samples to try immuno-FISH again (done) stain cells (done) finish letter and reply to Nicole about … Continue reading →

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Saturday 05/31/14

Posted on May 31, 2014 by admin

10:30a – 4:00p New probes to make: B10-B12 (last of the yellows) C09-C12 (last blue, first 2 blacks) D01-D07 (all the end-to-end blacks) [D08-D12 are Bogdan’s internal blacks] master mix 525 polymerase (25*21) 420 ddH2O 21 of Lib3 (1:50 dilution) … Continue reading →

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Friday 05/30/14

Posted on May 30, 2014 by admin

9:30a – 7:00p Goals Finish progress report limited-cycle RT PCR for new probes enter into database last batch of ~30 probes synthesized Analyze new 2-color data Literature interesting article by Fontana on aging driven by random-gene expression when lacking strong … Continue reading →

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Thursday 05/29/14

Posted on May 29, 2014 by admin

11:00a – 12:45a (9:00a-11:00a, volleyball practice). Meeting with Bogdan see notes Bogdan Data: \cajal\AlistairTemp3 Chromatin Data Analysis Chromatin Cropper Coding to do integrate radius of gyration computation integrate flip h/v/transpose options for conventional images fix auto-loading of conventional images (currently … Continue reading →

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Protected: chromatin data analysis

Posted on May 29, 2014 by admin

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Wednesday 05/28/14

Posted on May 28, 2014 by admin

10:00a – 11:00p STORM imaging F04F03 samples finish staining / washing B05 + new F03-F04 no secondary, F05-F06 no secondary (see labels on wells) cell staining Prep 12 new 18mm coverslips for staining Prep 3 new 40 mm coverslips of … Continue reading →

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Protected: project 2 update: 05/27/14

Posted on May 28, 2014 by admin

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