Author Archives: admin

Tuesday 05/27/14

Posted on May 27, 2014 by admin

10:30a – 11:45p Data analysis Launch STORM analysis for bead data crazy transpose now between conventional images and STORM images in F02F03 data. May affect all recent data? To do: Finish fellowship progress report 2 color imaging of other en-locus … Continue reading →

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Thursday, 5/22/14

Posted on May 22, 2014 by admin

10:00a – 10:00p Polymer sims periodic loops and sticky show more positive slope than just perodic loop model did (substantially). equilibrate slower. observations for yellow small 10-35 kb regions seem to be larger than expected by scaling extrapolated back from … Continue reading →

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Wednesday 05/21/12

Posted on May 21, 2014 by admin

11:00a – 11:30p Lab Volleyball, 9a-11a Meetings Project 2 weekly meeting, 12p-1:30p Meeting with Ting, 2p-4:30p Kc167 cells mostly tetraploid for all chromosomes (II, III and X). May vary in chrII but do not appear to in recent experiments. ~78% … Continue reading →

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Tuesday 05/20/14

Posted on May 20, 2014 by admin

10:00a – 10:50p Goals team meeting — need replies for XZ and Tani Stain new yellow domains troubleshooting multi-color Chromatin Analysis preliminary analysis of radius of gyration. wrote code to compute flists (now a Chromatin Function) for old data which … Continue reading →

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Friday 05/16/14

Posted on May 16, 2014 by admin

Chromatin Project Analyzing E06+ E07 data: chr2R:19726615-19888410__YELLOW_D12 a + b 4Tb hard-drive in Kangaroo mount died. All data from F03-F02 and F04-F02 lost. Drive recovery services probably not worth it. Damn it. chkdsk ‘recovered’ the files by deleting all the … Continue reading →

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Thursday 05/15/14

Posted on May 15, 2014 by admin

10:00am – 5:45pm Hal froze last night on movie 4! :(. Restarted hal, restarted imaging. Really should remember to check this before I leave — this must have happened before I went home last night near ~1 am, since the … Continue reading →

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Wednesday 05/14/14

Posted on May 14, 2014 by admin

8:45a – 12:45a 9:00a-11:15a, lab volleyball practice Probe synthesis B02-B09 3ul P1-A405, 10 uL RNA, plus 5 uL RT buffer, 2 uL dNTP, 1.5 uL ddH2O, 1.5 uL inhibitor, .2 ul RT Master = 27 uL P1-A405 + 45 uL … Continue reading →

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Tuesday 05/13/14

Posted on May 13, 2014 by admin

10:00a – 11:30p Project 2 see notes Chromatin Project New Probes making L3: B02 – B09 L2: F03, F04, F05, F06, F07. L3: F09, F10, F11 (all 2 color), running QPCR (try 2) over-ran a little bit, amplification happens quite … Continue reading →

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Protected: project 2 update: 05/13/14

Posted on May 13, 2014 by admin

There is no excerpt because this is a protected post.

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Monday 05/12/15

Posted on May 12, 2014 by admin

9:30a – 5:00p, 10:30p – 12:00a Goals Finish stains 2-clr STORM imaging En TAD regions Group meeting prep. Communication email Graham email Steph dates Lab Meeting (laptop battery died, no e-notes). 750 with adaptive optics? Probe making RT-PCRs didn’t work. … Continue reading →

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