Author Archives: admin

Thursday 08/22/13

Posted on August 22, 2013 by admin

10:10a – 7:00p, 8:50p – 12:10a STORM & staining BX-C tiles AbdA labeled coverslip (S2 cells) found cross-hatching imaging additional cells record chromatic beads will restain AbdA-stained coverslip with BX-C Ubx labeled coverslip (S2 cells) found cross-hatch (much more evident … Continue reading →

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Wednesday 08/21/13

Posted on August 21, 2013 by admin

9:45a – 11:30p cell staining heating buffer. wrong buffer, no formamide in 60C hot wash. Did I use the formamide buffer yesterday? I recall heating a 50mL tube and I don’t think I have 2x SSCT in a 50mL tube… … Continue reading →

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Tuesday 08/20/13

Posted on August 20, 2013 by admin

9:45a – 5:15p, 10:00p-12:25p General stuff mailroom still closed (10a) still can’t pick up package need to get PCR tubes, razor blades and 10x PBS from stock room. cell staining hot wash 65C in waterbath 17 min. no staining (low … Continue reading →

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Monday 08/19/13

Posted on August 19, 2013 by admin

9:30a – 8:30p Banff to do email speakers who haven’t sent in titles (individual emails sent). update schedule on website with tentative speaker order both goals complete for today Probe making oligo cleanup of probes new gel from O/N gel-green … Continue reading →

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Sunday 08/18/13

Posted on August 18, 2013 by admin

12:20p – 7:00p, 8:40p – 11:30p Goals reread and edit review analyze latest round of BXC data Probe making verify direction of MYcroArray BX-C Hox probes: probe region is “+” strand (antisense) T7 is on the nick side primer –> … Continue reading →

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Saturday 08/17/13

Posted on August 17, 2013 by admin

10:30a – 10:30p Helping Hao align FRET Wrote SplitQVdax.m SplitDaxFRET_130817 Note: splitting large dax files is slow and memory intensive. Even 2000 frames takes a few gigs in the memory map approach. (10:30-2:30). Will need to help get setup with … Continue reading →

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Friday 08/16/13

Posted on August 16, 2013 by admin

9:15a – 1:00p, 3:00p-12:00a Review Finalize figure 1. Encorporate all figure elements for examples section into figure 2. First pass design of new figures complete. Need to apply for permission to reuse images from Raj 2010 Nature, Frankel 2010 Nature, … Continue reading →

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Thursday, 08/15/13

Posted on August 15, 2013 by admin

9:45a-6:30p Review first attempt at discussion written (final paragraph still needed, and abstract needs revision). Working on figures. Notes for abstract / discussion (1) There are a handful of papers that arrive at interesting inferences about stochastic gene expression using … Continue reading →

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Wednesday 08/14/13

Posted on August 14, 2013 by admin

9:30a – 7:00p, 8:30p-10:45p (volleyball 9:50a-11:00a). Project 2 see post. Cell culture passage Kc cells preparing new medium: thawing and heat treating FBS Brian has Clone8 cells ready to go. Review wrote draft of section on PLoS CB paper: focus … Continue reading →

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Protected: project 2 08/14/13

Posted on August 14, 2013 by admin

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