Author Archives: admin

Tuesday, 08/06/13

Posted on August 6, 2013 by admin

9:45 A – 7:00 P, 8:30 P – 12:15 A Meeting prep STORM analysis analyzing temperature data about 75% fewer localizations at 52C than 47C little change in background / off-target localizations Running automated analysis of color data Automated analysis … Continue reading →

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Monday 08/05/13

Posted on August 5, 2013 by admin

9:40 A — 6:20 P, 8:00 P – 11:30 P Chromatin Paints, Library 2 finalization. Updates to code fixed bug in matching region names to sub-libraries fixed bug in screening for nick sites. Error checking spot check Drosophila sequences by … Continue reading →

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Sunday 08/04/13

Posted on August 4, 2013 by admin

10:00 A – 6:45 P, 8:30 P – 11:30 P Ph project washing out primary antibodies stain with new secondary antibodies. wash out secondaries Prep samples for STORM STORM O/N imaging of Ph-wt Ph-cy7 + Pc-647 750 mosaic acquired at … Continue reading →

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Saturday 08/03/13

Posted on August 3, 2013 by admin

9:50 A -7:30 P, 8:30 P – 12:00 A Transcription Modeling Worked out Feynman-Kac approach to transcription cycles see Mathematica notebook: TK_TxCycle.nb verified now by FK, S, and logic method that effect of gestation is minimal on reducing promoter noise … Continue reading →

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Friday 08/02/13

Posted on August 2, 2013 by admin

8:50 A -8:00P Discussion project 2 back up strategies blobology and the adventage of multicolor Ph project label antibodies start staining 6 coverglass samples of Ph-wt accidentally skipped borohydride treatement (doesn’t seem to have too much effect) skipped Triton-X permiabilization … Continue reading →

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Thursday 08/01/13

Posted on August 1, 2013 by admin

9:45 A – 7:25 P, 8:30 – 11:00 P Modeling Peccoud’s solutions for the two state model do have the correct form as backward reactions go to zero. check these solutions with Sanchez’s approach. Also still correct En embryo data … Continue reading →

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Wednesday 07/31/13

Posted on July 31, 2013 by admin

9:45 A – 7:30 P, 8:00 P – 10:00 P Discussion discuss bivalent domains with Alex Schier. read Vastenhouw & Scheir review. Schier group lab meeting tomorrow 3:45 STORM finish O/N run of Ph-Wt-flag-cy7, Pc-647 start analysis of en data. … Continue reading →

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chromatin paints activator tests

Posted on July 31, 2013 by admin

Possible matches: * X-1, cy5-2 * 0-1, cy5-2 * 405-1, cy5-2 * cy5-1, cy5-2 * cy5-1, X-2 Blind-Analysis Round 0 Round 1 R1S1 0-1, cy5-2 R1S3 405-1, cy5-2 R1S4 cy5-1, X-2 R1S5 cy5-1, cy5-2 R1S6 X-1, cy5-2 Round 2 R2S1 … Continue reading →

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Tuesday 07/30/13

Posted on July 30, 2013 by admin

9:30 A – 5:00 P, 9:00 P- 12:00 P STORM set up O/N Ph-wt Pc-cy7 Modeling Using renewal equations which say fano factor of time is eta^2 of transcript count variability (covN) should go like 1/kf for all N for … Continue reading →

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notes from analytical exploration of effects of chain length on mRNA variability

Posted on July 30, 2013 by admin

The details of this approach are not valid, since we require reversability to use this form for the variance. excised text from review draft: % Using Sanchez Approach Since only one state in the gestation cycle is transcriptionally competent, all … Continue reading →

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