Author Archives: admin

Tuesday 07/09/13

Posted on July 9, 2013 by admin

9:30 A – 7:00 P, 8:15 P – 11:00 P chromatin got 10 kb segments to run. Still lots of crashes, some substantial regions lack probes if we’re fragmenting sections so we have to stitch backtogether, might as well do … Continue reading →

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Monday 07/08/13

Posted on July 8, 2013 by admin

9:30 A – 7:00 P Lab meeting Presentation Notes Journal Club Notes Quick Project2 group discussion. Uploaded presentation stub for Tuesday Ph project quick look at recent STORM data performing chromatic bead analysis to align color channels. Psc PhM storm2 … Continue reading →

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Protected: Ph Data Pc Psc effects

Posted on July 8, 2013 by admin

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journal club 07/08/13

Posted on July 8, 2013 by admin

Steven presenting CRISPR cell paper One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering Background CRIPR repeat sequences separated by spacers. Spacers are different, match different bacterial phage sequences. components: Cas1, Cas2, Csn2 (involved in encorporating … Continue reading →

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Protected: lab meeting 07/08/13

Posted on July 8, 2013 by admin

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Saturday 07/06/13

Posted on July 6, 2013 by admin

Working remotely from Wareham. chromatin project HoxD project BLASTed hoxD probe sets against Drosophila genome. Haven’t even downloaded mouse genome Download and build BLAST+ and LegacyBLAST libraries for mouse genome. Screen primers against mouse genome some loci still refuse to … Continue reading →

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Thursday 07/04/13

Posted on July 4, 2013 by admin

10:30 A – 4:00 P project 2 planning meeting, layout update plan for Tuesday STORM analysis running Pc and Psc data on Cajal copying remaining Psc data to ProRAID Troubleshooting Chr2p75,364,833-75,411,965teloE2p1toE2p2_oligo won’t run in OligoArray runs at size 100 (not … Continue reading →

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Wednesday 07/03/2013

Posted on July 3, 2013 by admin

9:30 A – 6:30 P, 8:30 P – 10:00 P STORM 4 issues with recent software update troubleshoot with Hazen. Now running. If Dave/Steve freeze on STORM4 need Hal restart running O/N imaging of PhF Psc. STORM 2 took some … Continue reading →

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Tuesday 07/02/13

Posted on July 2, 2013 by admin

10:00 A – 6:15 P, 8:00 P – 10:30 P Adaptive optics for deep imaging Imaging with Shu lamina features not that sharp try using beads ontop of embryo for feducial calibration try staining AATAT probe or RNA in situ … Continue reading →

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Protected: project 2, 07/02/13

Posted on July 2, 2013 by admin

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