Category Archives: Summaries

Seminars Alex Pollen seminar (see notes) Microscope building HHMI equipment cycle equipment granted was 3 MPB lasers (560, 647, 750), Hamamatsu camera, Nikon microscope body w/ 8K of extras, Coherent 488 laser submitted separate quote addressed to Harvard for optics … Continue reading →

9:00 am – 5:00 pm Tasks lab meeting 10 am – 12:30 pm (see notes) FISH with Hazen — prepped cells up to hyb step, should be in formamide by tonight Reply to request from Biteen lab for code help … Continue reading →

9:30 am – 9:00 pm Tasks reply to MIT IMES / Physics reply to Caltech submit patent signatures Discussion with Hsuan – microscopes and plans Discussion with BB using common label as a fiducial for XYZ corrections validate if I … Continue reading →

10:07 am – Tasks Discussion about super-resolution of individual membrane proteins (EL lab). Reply to request for data from JM Victor. Project updates with BB. Need to contact LM team about explaining model. Discussion on quantifying nuclear structures with new … Continue reading →

9:30 am – communication discussion with Paul on MERFISH Microscope building updating parts list discussion with Hsuan on plans see shared dropbox folder and shared excel files (need to link in) Interview Prep prep for Salk and MCB interviews

10:00 am – 5:00 pm Probe synthesis start synthesizing 2kb probes (with SW secondaries and Fast secondaries) running T7 reaction O/N. To do test SDS treatment of embryos test 2 kb probes (scope time on Friday evening). Results from yesterday … Continue reading →

12:00 pm – 6:30 pm (10:00 am – 12:00 pm working remotely on library). Library prep updated 15 kb en library to use F primer L6F5 with L6R2 instead of F1, which I already used in lib7 ordered en 15 … Continue reading →

8:45 am – 10:40 pm schedule Rinse out probes, set up primary hybe for bit 1 for embryo staining experiment tracing BX-C Wu lab group meeting meeting with Scolnick at Broad Garner seminar on academic advice (see protected notes) continuing … Continue reading →

9:05 am – 7:30 pm, 8:15 pm – 11:40 pm Imaging testing control L4E24 embryos. stains look great. compare to L7 embryos — no staining. ran series removing S2 with toe-hold 5 bp. Looks excellent. Troubleshooting Gel tests try to … Continue reading →