Category Archives: Conference Notes

Quantitative measurements of transcription in single cells have demonstrated a high degree of stochastic variation among clonal cells in copy number of mRNA and protein, resulting from the bursts of molecule synthesis.  These experiments have raised the question of how … Continue reading →

Plenary Session II. Kami Ahmad (Harvard Med School) Nucleosomes. Histone replacement (H3.3). Targeted to active regions. Xnp part of replacement complex. H3 strongly expressed in S phase, H3.3 is constituitive. Constitutive H3 rescues all H3.3 phenotypes (sterility, lethality, etc) Process … Continue reading →

Pare mRNA counting in Drosophila (McGinnis lab) Recommend acetone permialization. No prot K, need longer hybe. Direct label not as sensitive, much more expensive. More As = better probe (but repeats give background)! Arney et al nut acids 2010. Mounting … Continue reading →

Techniques Platform. Perrimon group/ RNAi centers Cell based RNAi screening. Increase in imaging screens (from plate-reader screens). 1-3 million images per std experiment, taken an automatically analyzed. 2-10Tb per screen, currently 35Tb of data. Trying to move to in vivo … Continue reading →

Regulation of Gene Expression Part I Lehmann lab FACs and RNA-seq to study germline transcription. 7K out 13.8K genes transcribed in germline. Maternal to zygotic transition at stage 8-9 in germ cells. Ovo motif C-GTTA Zeba (dePace Lab) quantitative input … Continue reading →

Maggert lab, (Texas A&M) Ctcf regulates Erna synthesis in nucleuolos spatial and temporal dynamics of homologous recomb reapir.  (Karpen lab) double strand breaks in heterochromatin/ rpt dna. Double strand breaks peak earlier in heterochromatin. after irradiation expansion of hp1a Margarete … Continue reading →

Greg Reeves (NC State) Dorsal specifies boundaries of vnd, sog, dpp, etc in a concentration dependent manner. dorsal possibly too flat late for sog. Live imaging with lightsheet microscopy and dorsal-venus fusion requires only 1 copy to fully compliment doral … Continue reading →

Jerome Cartry (Bodmer lab, Sanford-Burnham) Let7 miRNA knockouts have cardiac arrhythmia. 3 target genes change expression levels. Wan Yoon (Montell Lab, Hopkins) Mir279 loss of function leads to extra border cells, similar to extra jak/stat signaling.

Fly Meeting: Larry Sander Lecture Pain response hypersensitivity (in healing) – nociception can become chronic (fibromyalgia, cancer arthritis) Allodynia (respond to previously non-irritant stimuli) hyperalgsia respond more seriously to minor irritant dronc activity (cell death response) is required for allodynia. … Continue reading →