Monthly Archives: January 2011

Fly stocks

Posted on January 20, 2011 by admin

Flip all: 2x MG-alpha into new bottles fog virgins into new bottles (for collection after Gordon conference). New cross: fog virgin x hkb:fog2 (orig. stock #M25). water all fly crosses.  Fog rescue crosses still look infertile (not surprising with 1:25 … Continue reading →

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Wednesday 01/19/11

Posted on January 19, 2011 by admin

7:30A – 4:00P, 7:20P – 10:10P Advanced Imaging Methods conference: (Today FLIM/SLIM and Live imaging) . See post on notes. Water fly crosses, flip collection plates.

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Notes: Advanced Imaging Workshop 01/19/11

Posted on January 19, 2011 by admin

Notes (taken on phone, sorry about poor formatting). FLIM lifetime imaging ave time before photon release.   10E-9s adv. sep. near spectra.    sensitive to ph etc.   independent of intensity in or out.   use w/ FRETmeasure distance.   can id distribution of distances … Continue reading →

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Protected: hb-enhancer modeling cont pt2

Posted on January 19, 2011 by admin

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Protected: hb enhancer modeling cont…

Posted on January 19, 2011 by admin

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Tuesday 01/18/11

Posted on January 19, 2011 by admin

10:30A – 7:00P, 8:00P-12:10A Need to prep for Advanced Imaging Methods Workshop tomorrow through Friday. (starts 7A tomorrow). Fix embryos, flipped bottle stocks. Enhancer modeling: Adding explicit biochemical mechanisms for enhancer reading.   Previous method had no restriction from molecular … Continue reading →

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Common git commands

Posted on January 18, 2011 by admin

Making notes of common git commands: From within the file directory (PATH$),  remove files which have been deleted: $(git ls-files –deleted) delete all removed files, update whatever was modified, add new files $git add -u [Neither of these remove from … Continue reading →

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Fly Work

Posted on January 18, 2011 by admin

Flip collection cages, 2x mat-a Gal4 x UAS-twi2, UAS-da, UAS-m8: 10:50A Flip collection cages, 2:00P, Fix embryo, 3:00 P Flip bottle stocks, add foam lids to bottle stocks and two working flats of bottles.

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Monday 01/17/11

Posted on January 17, 2011 by admin

10:20A – 7:30P, 8:20P – 11:30P Finishing touches on lab meeting presentation. Extend analysis to kr, kni, and snail enhancer data.  Assume enhancers similar to hb and then see how different they need to be to explain data.  Data is … Continue reading →

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Protected: enhancer modeling

Posted on January 16, 2011 by admin

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