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Monthly Archives: January 2011
Fly work
Found 2 more fog rescues males. (for 3 out of 109). (Expect 1:2). Collect UAS-twi-twi males and cross to 2x Gal4-ma virgins. Collect UAS-da males and cross to 2x Gal4-ma virgins. Collect UAS-Espl males and cross to 2x Gal4-ma virgins … Continue reading
Posted in Fly Work
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writing
Working on text and figures for “Effect of binding site number and spacing on transcription regulation” MS. General outline: Recap key conclusions from Gregor and Dostatni papers. Motivate developing a method for relating transcriptional activity to limits in concentration (integrated … Continue reading
Posted in Transcription Modeling
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Gordon Conference Prep
Abstract for Gordon Conference: Genetic manipulation is increasingly totted as the solution to the major challenges of our generation; combatting non-infectious diseases like cancer and heart disease through gene therapies, finding new sources of global energy through bio-synthetic microbes, or … Continue reading
Posted in Research Planning
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Protected: Progress Report
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Protected: hb enhancer modeling
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Posted in AP patterning, Shadow Enhancers, Transcription Modeling
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PoSB magazine finished
Volume 1 of Principles of Synthetic Biology now live. http://alistairboettiger.info/PoSB_Mag_v1.pdf
Posted in Teaching
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Fly work
Collect virgin w;Sp/CyO;Hb[D]/GFP fog- 2x MG-alphas Dostatni will send: stock that has 4 insertions of the pnanos-Gal43GCN4-Bcd3’UTR transgene (1 on each 2nd chromosome and 1 on each 3rd chromosome). Lab fedex account is closed. Kevin will set up an … Continue reading
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Fly work
Collect virgins, w;Sp/CyO;Hb[D]/GFP and 2x MG-alphas (9:30 A) Collect virgins, w;Sp/CyO;Hb[D]/GFP and 2x MG-alphas (9:00 P) Flip fog into new bottles to expand. Start collecting virgins tomorrow. w;Sp/CyO;Hb[D]/GFP have mites. Need proper lids (probably acquired during break when old food not … Continue reading
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Comparison to Gregor data
To count all 770 molecules in the nucleus according to Thomas data (D =1, c=4.8, a=.003) would take 890 minutes (and give 3.6% diffusion error). naturally 100 molecules gives a std/mean 0f 10%, and is thus the natural threshold for … Continue reading
Posted in AP patterning, Transcription Modeling
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Protected: Comparison to hb data
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