Daily Archives: February 7, 2011

Monday 02/07/11

10:10 A – 7:00 P, 8:00P – 11:00P Image processing: add multichannel capacity to im_singlemolecule.fig, apply to sna BAC and ths/sog as first pass Fix embryos (2xMG-alpha x LacZ on II, and MP08 in sna[D]. Phil lab meeting miniprep yellow(i) … Continue reading

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Image Processing

Added multi-channel capability to im_singlemolecule.fig Added slice monitoring steps MP10_sna_y_22C emb 02-05 all start too deep in embryo, miss transcript.  06-10 no data. MP10_sna_y_22C_b 02-05 also too deep, 06-10 no data. MP05 data is good ths-dnp is pretty marginal. Time … Continue reading

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Probe and Plasmid building

miniprep y-full length and y-(i) plasmids Set up PCR for probe templates: y-full, y(i), hb(full)

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Fly work

Flip collection cages, 10:30 A water crosses, (male 04; Pr x DGTG are failing/dying).  Other 04s look okay. Flip collection cages, 1:15P

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