Daily Archives: February 1, 2011

Improving dot z-segmentation

Posted on February 1, 2011 by admin

Improving z-dot segmentation double checking code: Turning mRNA counts into graphs.  With correcting for cell size variation gradient appears smoothly.  (I plot instead mRNA density per cell, and multiple by the mean cell volume to return to normalized total counts … Continue reading →

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Tuesday 02/01/11

Posted on February 1, 2011 by admin

10:00A — 7:00P, 8:00P-12:00A Mendeley Berkeley Advisors meeting Jen Confocal UAS x Espl-cluster genes affects on snail patterning New slide for ML: median embryo from each population showing multiplicative action. Planning in situs for later this week. Integrating new duplicate … Continue reading →

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Understanding bursty Gal4-LacZ expression

Posted on February 1, 2011 by admin

Researching UAS-LacZ line sequences to probe stochastic expression effects. my UAS-LacZII from flybase stock 3955, From Kathy Matthews, 1998 gift of flybase. contains: P{UAS-NZ}20b — SV40 T NLS-lacZ fusion in pUAST (beta-gal is localized to the nucleus). Insertion on chromosome … Continue reading →

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