Monthly Archives: July 2011

Saturday 07/23/11

5:00P – 6:30P, 8:00P-9:00P Setup and run confocal of MP07Hz snaD embryos

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Friday 07/22/11

9:00A -12:30P mRNA counting (see post) numbers synthesis rates, decay modeling sent to ML Discuss merits of mathematical formulation of snail boundary bistability with Jacques. Proofs back for PNAS paper on gap-shadows. Fix yellow shade in fig 2D legend, review … Continue reading

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Protected: mRNA snail numbers and models

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Thursday 07/21/11

8:15 A – 4:50 P Finish ON confocal sna2.8 30C hets Start confocal twiN vnd, sna.  vnd is expressed in twi null twiN sim, sna imaging.  sim is expressed in twi nulls Working on plotting rescue data with new added … Continue reading

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Protected: sim sna thresholds

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Wednesday 07/20/11

9:00 A – 3:45 P, 8:00P – 9:50P, 10:50P – 11:45P Finish ON confocal of MP08Hz_snaD_b rescues ON dotfinding of MP12Hz_snaD was computed on single channel only. Need to repeat. Write to Banff participants about carpool (Mary, Gregor, Narendra, Brian).  Need … Continue reading

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Tuesday 07/19/2011

8:30 A – 7:00P, 9:00P – 10:40P Finish ON confocal MP12Hz with sna/sim Start new confocal fun MP06 channel-flip Update probe database: ANB_Probes.xls (excel 95 format for Mac-Matlab excel import to work).  Match all existing probes and probe templates. Update … Continue reading

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Monday 07/18/11

8:20A Projecting 2.8het sna sim counting data Analyzing 2.8het sna sim data Projecting MP12 Hz data Analyzing MP12 Hz data Confocal MP05Hz repeat (start warmup 2:15). Finish flipping stocks except APT eye screening 3 new bottles of AP-toll Lab meeting … Continue reading

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Sunday 07/17/11

3:ooP Finish ON confocal, vertical drift lost most of stack of MP06 Hz embryos :(.

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Saturday 07/16/11

7:00 P — 10:00P Setup ON confocal, MP05Hz (from 6/20) offset 8.5 um, warmup 1.25 hrs, still drifted.  Maybe too long refinding embryo planes and running sample scans.  use more aggressive vertical offset (5 or 6).

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