Monthly Archives: October 2012

Tuesday 10/16/12

9:00 A – 6:50P, 8:20P -11:15P discuss CMAKE minGW sys with JM 9:30 A flip collection plate Working on BioPhys J review. 11:30 A – check samples from oven substantial issues with resin quality: 1 sample R solid at 70C. … Continue reading

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observations on Insulator organization

CTCF sites are TF rich: (en locus) CTCF sites are TF rich ~HOT DNAs?  (though not as strong DNA-hypersensitive regions).  rich ~HOT DNAs?  (though not as strong DNA-hypersensitive regions). Do these insulators co-localize (most insulators HiC/3C together) Coerces 2012 MolCell chromatin domain boundaries … Continue reading

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Monday 10/15/12

9:30 A – 7:30 P Flip collection plate on MTD flies.  starting to lay…. sorta. looking at aptamers for histone labeling (see Mendeley). DNA-FISH + protein prep for STORM protocol test try 2:  samples now embedding. [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 302 sheet=8] … Continue reading

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Protected: Analysis of En locus k27me3

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Protected: Pc clustering over time (at SR)

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Protected: Pc and K27me3 dual labeled first analysis

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Protected: K27me3 density

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Matlab Coding guidelines

  On writing readable matlab code [In development] Rule 1: The code you run and re-run on different stuff should be functions Functions can be shared / read used by others.  Scripts generally cannot.  Script with front-matter inputs = bad … Continue reading

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Sunday 10/14/12

10:20 A Fly work New cage of MTD (not crossed, just as control).  Use for Pc and Su(Z)12 staining.  Methanol treat after primaries to improve in situ. DNA FISH Day 3: DNA-FISH + antibody. [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE sheet=8 602 302] postfix 1 … Continue reading

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Saturday 10/13/12

11:00A – 6:ooP, 9:ooP-11:00P binomial statistics and confidence intervals discussion with JM Collect virgin E(spl) and sim[D] flies, 4 more vials of crosses. Finished virgin collections and cross-setups for present.  All flies now on RT shelf.  Organizing Matlab STORM library … Continue reading

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