Monthly Archives: November 2012

Friday 11/30/12

Posted on November 30, 2012 by admin

9:30A – 10:15 P Fly Work Collect sna[18] x Double balancer virgin and male F1s PM virgin collection STORM2 time. Colenso removing gas laser from STORM2 Mount ultra cryo section embryo for imaging sections not flat or hard to find. … Continue reading →

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Thursday 11/29/12

Posted on November 29, 2012 by admin

9:20 A — 7:15P, 8:15P – 10:00P Polytene cell Check polytene cell staining.  Faint glow of nuclear laminin visible.  pretty dim, does not have ring shape, see top and bottom (would be easier to distinguish as diffinitive stain by confocal). … Continue reading →

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Wednesday 11/27/12

Posted on November 28, 2012 by admin

10:15 A — 11:15 PM Polytene cell staining  hot washes in 2x SSCT (45 C in coplin jar in dry oven) check staining — cent probe looks good (rather small foci though) Antibody staining H3K9 did not work.  Should have … Continue reading →

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Tuesday, 11/27/12

Posted on November 27, 2012 by admin

9:40 A – 8:00P, 9:15P — 11:40P Fly Work collect virgin 2x HbZ. clear MTD x Pc1 parental generation. check Espl x sim crosses write to Maria to cancel Wed sectioning test gel PCRs No substantial bands New gel: Espl-DNA … Continue reading →

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Monday 11/26/12

Posted on November 26, 2012 by admin

9:35 A – 7:00P, 8:20P – 11:50P STORM chromewarp 3D has much more trouble vertically matching beads when we take the same field for each z.  Need to change this.    1 manual edit of the beadrun.xml file ought to … Continue reading →

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Sunday 11/25/12

Posted on November 25, 2012 by admin

11:00 A – 5:00P, 8:00P – 10:00P Code development Working on new Matlab tool for rapid color channel adjustments to images and drag and drop image loading capabilities (faster and more convenient than doing color switching in photoshop). GUI is now in … Continue reading →

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Saturday, 11/24/12

Posted on November 24, 2012 by admin

11:15 A -7:30P, 9:30P – 11:45 P UltraCryo coverslip DFISH Hot washes for DFISH coverslips RT wash check sections on coverglass A.  Be very careful to keep sections covered with buffer. Staining definetely works.  Signal somewhat dimer than expected, but … Continue reading →

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STORM2 11/24/12

Posted on November 24, 2012 by admin

Laser powers 405: 1.5 mW 488: 115 mW at 45% power 561: 135 mW at 200 mW GUI/laserhead 642: 135 mW at 200 mW GUI/laserhead 750: 250mW at 1800 mA. –> too high? 750: 120 mW at 1400 mA –> … Continue reading →

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Friday 11/23/12

Posted on November 23, 2012 by admin

11:00 A – 2:00P, 7:00P – 10:45 P probe making: precipitate, wash, resuspend and freeze probes. Ready to try. Cryo-section staining secondary stain and wash of DFISH coverslips No sections remaining on DFISH coverslips.  Try crosslinking? staining on coverglass does … Continue reading →

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Wednesday 11/22/12

Posted on November 22, 2012 by admin

9:10 A — 8:00P, 10:30P – 12:45 A UltaCryo collect prepped samples 10A – 2P, cutting ultra cryo sections of 1-10hr MTD DFISH-fixed embryos For next time: denser embryo packing in smaller gelatin sections Cryo protect at least 30 min … Continue reading →

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