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Daily Archives: November 19, 2012
Monday 11/19/12
9:15 A — 7:15 P, 8:15P – 1:15 A Cell labeling hot washes for probe first slide (2x at 60C) 1x at RT remove and save primary antibodies at RT for primary first slide. PBT rinses (3x soln change, 3rd … Continue reading
Posted in Fly Work, probe and plasmid building, Summaries
Tagged cell labeling, probe making, snail, STORM2
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Protected: Notes – Lab meeting 11/19/12 Graham
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Posted in General STORM
Tagged lab meeting, notes
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STORM2 rebuild SOP
Follow text file SOP for 750 laser. Aligning back optics place microscope in epi mode (12.000) Course tuning remove 20x, 100x objectives. screw in target objective in 100x objective position. remove final lens before scope body, add iris in … Continue reading