Monthly Archives: October 2012

Wednesday 10/31/12

Posted on October 31, 2012 by admin

9:45 A – 8:40P, 9:45P-11:45P Submit revised snail kinetics paper. Collect virgin Espl/sim cross to TM2/TM6 Collect virgin Act5 Gal4.  Cleared and added new bottle of these to virign collection. washout Pc antibody from O/N MTD post fix 5 min … Continue reading →

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Tuesday 10/30/12

Posted on October 30, 2012 by admin

9:30 A – 7:30P, 8:30P – 11:50 P Flies Flip collection plates. # Save MTDs to plate into new bottles.  All in one, need high density. Collect virgin Espl/sim, cross to TM2/TM6 males. Collect virgin Act5-gal4 Make new AJ plates … Continue reading →

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Monday 10/29/12

Posted on October 29, 2012 by admin

10:40 A – 8:15 P lab meeting canceled due to hurricane Sandy contact ML about revisions. Got suggestions for graphical abstract, now implemented.  Waiting for response on text. contact Ting about T7 primers Plan to meet with Brian and Fred (Thursday? … Continue reading →

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choosing insect cell lines for chromatin STORM

Posted on October 29, 2012 by admin

Info on adhesion. From: http://www.flyrnai.org/DRSC-PRC.html.  Also has protocols for maintenance.  Table shows cell line, growth at RT, recommend dilution when splitting cells. A. Semi-adherent cell lines – will stick to new flask but come loose over time S2* 3-4 days 1:3 – 1:4 … Continue reading →

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Sunday 10/28/12

Posted on October 28, 2012 by admin

10:30 A – 1:45P, 4:45 – 8:15P Fly Work 10:30A collect virgin Espl/sim 12:00 P, flip collection cages 7:30 P, fix embryos, 0.5 – 7 hrs MTDs, Psc 1, and Pc 3.  Left on shaker.  Good embryos should be sunk … Continue reading →

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in situ update

Posted on October 28, 2012 by admin
[spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 302 sheet=8]
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Saturday 10/27/12

Posted on October 27, 2012 by admin

10:30A – 8:ooP Stop O/N confocal by 11A. Confocal data check: Late positions start too low.  Not sure why — maybe middle position to close to edge changed microscope positions? Fly Work Collect virgin Espl[D]/ sim[D].   Flip fly stocks … Continue reading →

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Friday 10/26/12

Posted on October 26, 2012 by admin

10:20 A – 7:30PM Fly Work  Set up collection cages for TRiP knockdown experiments: MTD x Psc 1 MTD x Pc 3 Set up new collection cage of MTD controls.  Pc labels looked weak and Pc in situs did not … Continue reading →

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Thursday 10/25/12

Posted on October 25, 2012 by admin

9:50 A – 7:00P, 8:30P – 11:20 P Clean and coat coverslips washout fix from O/N fix samples, move to EtOH rehydrate, check for presence of staining. Respond to team about NSF BIRS proposal Pol I arrived 4:00 PM check … Continue reading →

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Wednesday 10/24/12

Posted on October 24, 2012 by admin

9:15 A – 7:00P, 8:00P – 12:00A RNA FISH + Immuno-stain in cultured cells (developing protocol:) warming up hybe solution for hot washes remove, label and save probes (in yellow -20C dilute probes box, labeled as S2). 3x Hot wash … Continue reading →

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