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Daily Archives: November 15, 2012
Thursday 11/15/12
9:15 A — 6:45 P, 8:30P – 12:00A collect virgins for sim Espl[D] recombineering new cross, sna males x Sp/CyoZ; TM2/TM6 virgins clear MTDs and TM2/TM6s for virgin collection (peak emergence for latest bottles?) pulverize and refreeze Espl[D] and sim[D] … Continue reading
Posted in Fly Work, probe and plasmid building, Summaries
Tagged fly work, probe making, writing
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issues with non-blinking weak spots
The very bright, round foci clearly do not correspond to objects in the conventional image and are probably all artificats from non-switching foci. These dots start off at moderate single molecule brightness (can’t eliminate them just using higher threshold cutoff … Continue reading
Posted in General STORM
Tagged images, troubleshooting
Comments Off on issues with non-blinking weak spots