Daily Archives: December 15, 2012

Saturday 12/15/12

10:30 A – 7:00P Working on presentation Check split cells. S2R+ ~80% confluent, should be able to split tonight. Kc167 < 50% attached Genome browser Tubigen version of Galaxy not talking to UCSC.  Spent several hours troubleshooting workflow only to … Continue reading

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Research Planning 12/14/12

Use antibody accessability as a proxy for PolII accessability. Compare levels of staining of H3K27me3 in intact vs. mechanically spread nuclei.  (First we need to see if H3K27me3 even remains associated in spread nuclei.  Try spreading nuclei using the ‘shearing’ … Continue reading

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