Monthly Archives: January 2013

Thursday 01/31/13

Posted on January 31, 2013 by admin

10:45 A – 12:30 A (8:45-10:45 workout) embryo section labeling quick check after 10 min hot wash in incubator at 70C in SSCT, found no sections. DAPI staining sections. Try again. weak BXC detectable: Prepped for STORM TRY 2: prepping … Continue reading →

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Wednesday 01/30/13

Posted on January 30, 2013 by admin

9:30 A – 6:30P, 9:00P – 11:20P STORM Samples: BX-C cy5. Imaged 4 loci before 3P. Turned microscope over to Doory. Will setup to image more loci overnight Fab7 405, cy5. Image tomorrow night? Ubx Cy5. Store at 4C in … Continue reading →

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Oligo Paints Goals 01/30/12

Posted on January 30, 2013 by admin

In situs single color BX-C-cy5 in S2R+ cells (done) BX-C-cy5 in S3 cells (image: 01/30/13) Fab7-cy5 + 405 activator in S2R+ (image: 01/31/13?) Ubx-cy5 in S2R+ (?) Abd-B in S2R+ cells (not yet stained) Abd-B in S3 cells (not yet … Continue reading →

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Tuesday 01/29/13

Posted on January 29, 2013 by admin

10:00 A – 7:20P, 8:45P – 11:45P General As of Today, all IDT oligo orders are to go through Matt, not through HHMI card on file. Cell labeling heating up 2X SSCT wash to 60C in large hybe oven check … Continue reading →

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Monday 01/28/13

Posted on January 28, 2013 by admin

9:45A – 10:00 P Lab meeting (notes) Fly work scored 5 more back-cross lines (no rescues still):  [spreadsheet 0AjSqkxgziU1YdENuMzFjejM0TnNXUEQ1OWdHRW1OY1E 602 202 sheet = 1] Flipped and watered bottle cross of Df/CyO,esc[2] x Sp/esc[6] males. New bottle cross of Df/CyO,esc[2] x Sp/esc[6] males … Continue reading →

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lab meeting 01/28/13

Posted on January 28, 2013 by admin

Will, BPS practice talk ISWI remodlers can assemble arrays denov.  space nucleosomes out regularly — critical for silencing and folding leading existing model: loop propagation DNA binding domain scoops in 10 bp from entry side (feeds this into entry side). … Continue reading →

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BXC + H3K27me3 first try

Posted on January 27, 2013 by admin

  K27me3 post-in situ labeling turned out pretty sparse.  Still have a few thousand localizations for which cluster nicely in several cases at the BX-C locus.   Overall this 488 staining is very sparse, there are maybe an order of … Continue reading →

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Protected: BX-C clusters first look

Posted on January 26, 2013 by admin

There is no excerpt because this is a protected post.

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Coding goals ~01/26/13

Posted on January 26, 2013 by admin

Install Readme for DaoSTORM (which files do we need to copy) compiled binaries for 64-bit windows installation and filepath setup for MinGW-64bit See if Homotopy Install guide works.  If so similar approach should also get DaoSTORM running. New molecule list … Continue reading →

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Friday 01/25/13

Posted on January 25, 2013 by admin

(out sick.  Trying to work a little form home) STORM stop O/N STORM run of BX-C-cy5 / H3K27-488 Call Bender busy signal, try later (2x) ovoD line on chr2 not extant in Bender stocks Available alleles Psc h27, II, 48, … Continue reading →

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