Daily Archives: January 15, 2013

Tuesday 01/15/13

Posted on January 15, 2013 by admin

10:00 A – 7:00P, 8:30P-11:30P Probe making in vitro transcription note, spreadsheet is interactive, all reactant amounts aside from template DNA (depends on spec’d conc) computed by sheet. Coding working on new code (STORMfinder) to replace interactive selection of dot … Continue reading →

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synthesis of RNA in situ probes

Posted on January 15, 2013 by admin

Set up in vitro transcription reaction as follows: Reagent Volume 5x transcription buffer (ProMega): 4ul 10x hapten (Dig. Bio DNP) U-NTP mix 2ul 100mM DTT 1ul RNase inhibitor (RNasin) (50 μ/μl) 2ul linearized DNA (~250 ng if 0.2μg/μl) 1ul (vary … Continue reading →

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