Daily Archives: January 16, 2013

Research goals

Posted on January 16, 2013 by admin

Oligo Paints Week of 01/15/13: synthesis en-Cy5 probe (by my hands in Zhuang lab and by Brian’s trainee at HMS) and of BX-C locus (by Brian). Monday-Tuesday (01/21-22/13), test in situs of en and BX-locus Tuesday: section Drosophila yw embryos … Continue reading →

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Wednesday 01/16/13

Posted on January 16, 2013 by admin

9:30 A – 7:30 P  Finish comments on counting comparison for ML # Finish revisions Write to MERCK about Probe Making Precipitate and resuspend RNA-FISH DNP probes for tou, inv, en, Antp and Dfd. Make new RNA-Hybe soln to resuspend … Continue reading →

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RNA in situ hybe buffers

Posted on January 16, 2013 by admin

Hybe Solution 50% Formamide (Amresco, ultrapure) 5x SSC 100 ug/mL salmon sperm DNA 50 ug/mL heparin 0.1% Tween-20   Reagent For 250 mL stock Formamide 125 mL 20x SSC 62.5 mL Heparin 10 mg/mL lab aliquot 1.25 mL 10% Tween-20 … Continue reading →

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RNA in situ hybridization

Posted on January 16, 2013 by admin

 Day 1 Xylene clearing ~2 hrs Rock embryos in 1:1 MeOH:EtOH 5 min (Waste -> organic waste bottle) Rinse 2x with EtOH Rock in EtOH 5 min Remove all but 100 uL EtOH, Add 900 uL Xylenes 1.0 hr (embryos should … Continue reading →

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