Daily Archives: January 23, 2013

Wednesday 01/23/13

Posted on January 23, 2013 by admin

10:30 A – 8:30P, 9:30P – 11:45P (8:45-10:30A workout) Fly Work collect virgins: Df/CyO,esc[2],  2x mat-tub Gal4. collect males: esc[6]/Sp. cross (1 new bottle) esc[6]/Sp x Df/CyO,esc[2] (should have used Gla, will need to screen Sp for all flies that … Continue reading →

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DNA FISH on coverglass

Posted on January 23, 2013 by admin

Adopted from Wu lab protocol.  01/23/13 Briefly wash cells with 1x PBS (not PBT) Fix cells with 4% formaldehyde for 15 min Quick wash with 1x PBS Wash cells with 2x SSCT for 5 min Wash cells with 50% formamide/2x … Continue reading →

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STORM2 BX-C 01/23/13

Posted on January 23, 2013 by admin

Setup Back-optics aligned Focus lock working Laser power: 647 laser at 200 mW source: 130 mW at power meter 405 laser: max power = 1.89 mW.  10% = .09 mW z-stage drift without focus lock is ~800 nm per min … Continue reading →

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