Monthly Archives: March 2013

Sunday 03/31/13

10:30 A – 8:00 P Review writing fixing bugs in implementation of synergy analytical framework. Got Feller V2 from library. Verified that approximations in t are limits approached by convergence to equilibrium. So the steady state values production rate/decay rate … Continue reading

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Saturday 03/30/13

11:15 A – 8:15 P, 9:30 P-11:55 P Review working on explanation of individual site contributions to cooperativity: Jiang 1993 Lebrecht et al 2005 make Bcd rescue embryos lacking the Bcd interaction domain required for cooperativity in vitro. They observe … Continue reading

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Friday 03/29/13

9:30 A – 8:10 P, 9:00 P – 12:10 A Install cryo-attachment be sure to retract arm and center FC mount prior to mounting be sure there is space between the arm and the chamber so the arm can move … Continue reading

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Thursday 03/28/13

10:00 A – 7:00 P, 8:30 P – 11:30 P Review writing Durrett’s Essentials of Stochastic Processes has a 2nd edition now which is available online: Springer, and Durrett’s page Re-organzing introduction: Simple-models form simple orgnaisms challenges of multicellular organisms … Continue reading

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TJ Ha seminar

some short notes: DNA bending: requires 30 KT to bend 100bp circle. But lots of structures (e.g. nucleosomes) bend DNA on this scale. Looping time has a very weak length dependence. Weak dependence is not surface induced (watch individual DNA … Continue reading

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Wednesday 03/27/13

10:00 A – 7:00 P, 9:30 P – 12:20 A STORM Break down O/N STORM run on STORM3 of fab7-405-cy5 STORM2 has issues Iris for columnating optics is too small for 512×512 view Focus lock can’t get minimal signal TIRF … Continue reading

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Tuesday 03/26/13

10:30 A – 7:30 P, 8:30 P – 10:30 P Tx-review working on literature references, laying out introduction. Stinchcombe model write out probability mass function of discrete mRNA arrivals in terms of the random differential equation for the continuous mRNA … Continue reading

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Monday 03/25/13

9:50 A – 7:45 P, 9:30 P – 12:15 A Lab Meeting (notes) Journal Club: (notes) Research update meeting preparing update on multi-color DFISH No data on BX-C vs Flank, I recall it didn’t work well but I thought I … Continue reading

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Protected: preliminary BX-C embryo

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Journal club: Aberrant light directly impairs mood

Aberrant light directly impairs mood and learning through melanopsin-expressing neurons LeGates et al 2012 (presented by colenso) Circadian rhythm Natural pacemaker controls circadian rhythm. 50% of blind individuals experience ‘free running’ – no entrainment to light. about a 25 hr … Continue reading

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