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Monthly Archives: March 2013
Tuesday 03/12/13
10:00A – 11:15P STORM Finish O/N STORM run on STORM2 Finish O/N STORM run on STORM3 Installing mount and sliders on STORM4 to allow for 3D imaging Lessons: Final appeture (removed for alignment) front-back position chosen to minimize the size … Continue reading
Monday 03/11/13
10:00 A Group meeting (Eileen) see notes Journal club: High salt recruits aversive taste pathways STORM Hal crashed on STORM3 at 10:11A (still 34 locations acquired). sent STORM3 error report to Hazen (see email record) STORM2 run seemed to finish … Continue reading
Journal club: High salt recruits aversive taste pathways
Nature, recent Presented by Guisheng 5 classes of taste receptors for umami, sweet, bitter, sodium and sour and carbonation each taste bud contains different cell types containing different receptors tongue map is wrong — each tastebud has all cell types. … Continue reading
Posted in Journal Club
Tagged neuroscience
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Protected: lab meeting 03/11/13
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Posted in Lab Meeting
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Protected: Alternative library and probe designs
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Posted in Chromatin, probe and plasmid building
Tagged chromatin colors, probe design
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Sunday 03/10/13
9:30 A – 12:15 A Meeting with Ajaz oops it’s daylight savings day! Ajaz will induce and fix cells, bring over fixed cells on coverglass label with m-anti-Flag + A647-A405-dk a-m secondary and STORM. Compare clusters. STORM Finished O/N imaging … Continue reading
Saturday 03/09/13
10:30 A STORM setup imaging of 2x-Cy5 labeled Fab7. Hardly detectable loci in conventional — many (most?) cells no candidate locus detectable. General cy5 background seems higher. Found a few cells that look like reasonable candidates for conventional. Background clearly … Continue reading
Friday 03/08/13
10 A – 11:00 P Transcription models Finished derivation from yesterday on multiple binding wrote up in Review, started extension to cooperativity Markov Chains in TeX write like chemical equations with mhchem package Use Tikz drawing package Named Graphs (Tikz … Continue reading
Code updates
updating other changes in directory. Old files may get restructured here soon into subdirectories. + .gitignore + Perry2011PNAS/read_planeT.m m Unsupervised_DotFinding.m + imclean.fig m imnucseg_pars0.mat m imnucseg_pars1.mat + imviewer.fig + imviewer_lsm.fig m imviewer_lsm.m m jacquestiffread.m updating other changes in directory. Old … Continue reading
Posted in Microscopy
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Thursday 03/07/2013
9:30 A – 11:10 P Coding Reorganizing Image_Analysis Repository New folder for 2013 Cell Reports paper Major rewrite of LSM parsing code And 6 hours later: LSMextract.m – Takes a .lsm file, extracts all z-positions and all stage-positions into multicolor … Continue reading