Daily Archives: September 22, 2013

Sunday 09/22/13

Posted on September 22, 2013 by admin

5:30p – 8:30p Bogdan / Rotation project prep: move black IVT reactions to top of upstairs -20C freezer new DNA FISH in situs E6 (E(Pc) region, 15kb) F6 (Y/R 140 kb) Stain E6/F6 originals with 5 uL + 1 uL … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged | Comments Off on Sunday 09/22/13

Notes for deep sequences of libraries

Posted on September 22, 2013 by admin

Bauer Core equipment training option 1 Amplify sublibraries * Buy NEB DNA kit, end repair * blunt end ligation alternative PCR primer gel-extraction Qubit concentration (nanodrop?) [optional] BioAnalyzer at Bauer Core. [optional] qPCR, dilute sample a few times. By Illumina … Continue reading →

Posted in probe and plasmid building | Tagged | Comments Off on Notes for deep sequences of libraries

Friday 09/20/13

Posted on September 22, 2013 by admin

10:15a – 6:30p, 8:30p-10:00p Planning Discussion meeting with Ting 10:15a-11:15a (see notes) Planning embryo Pc next steps (see notes) Writing continue shortening BJ review Re-read and submit referee comments. Added extensive additional discussion on conclusions. Experiments with Bogdan Set up … Continue reading →

Posted in Summaries | Tagged | Comments Off on Friday 09/20/13