Monthly Archives: October 2013

Thursday 10/31/13

Posted on October 31, 2013 by admin

9:30a – 10:00p STORM Stop O/N STORM of E12: 630 kb G transfer data to ProBox 4 Running out of disk space again. Order 2x 4Tb HDs Set up O/N STORM G9 (75kb B) Rowland Inst (10a – 2:30p) Seminar … Continue reading →

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Protected: Drosophila Transcription Projects

Posted on October 30, 2013 by admin

There is no excerpt because this is a protected post.

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Wednesday 10/30/13

Posted on October 30, 2013 by admin

9:00a – 10:50p STORM Finish O/N E10 imaging Hao imaging set up O/N imaging of E12 (600 kb G) with 20:30,000 bead dilution. Qualitative observations: some G regions quite large, seem pretty decompact. Others more compact? Laser power dropped to … Continue reading →

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Ph Project To Do

Posted on October 30, 2013 by admin

Goals: Counts co-localized vs. not co-localized in Mutant vs Wildtype Psc knockdown and imaging More numbers for Ph in Ph-Wt and Ph in Ph-M Modeling: a. get correct random walk behavior from polymer model b. reproduce data trend with ka … Continue reading →

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Tuesday 10/29/13

Posted on October 29, 2013 by admin

9:30a – 10:00p Other Goals Spoke broke. Pick up repair between 4p-6p. Presentation Prep working on slides for Rowland Center Talk. STORM Finish O/N imaging of E08 (75kb R) Set up imaging of E10 (100kb Y) Mentoring HC genes for … Continue reading →

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Monday 10/28/13

Posted on October 28, 2013 by admin

10:00a – 8:30p Goals reboot Tuck/Cajal disconnect all external drives and power supplies. Clean-up all cables for Tuck/Cajal. Check STORM4 run. Work on Slides for Rowland Center Talk on Thursday. Run genes for Hao STORM4 Lost focus somewhere. Looks like … Continue reading →

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Sunday 10/27/13

Posted on October 27, 2013 by admin

5:30p – 10:15p STORM Record z-calibration scans for STORM4 Imaging E1 (200 kb green) cells on STORM4 Chromatin modeling Found small bug in Bogdan’s code which prevents condensation This seems to now to work as expected. Data copying data from … Continue reading →

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Saturday 10/26/13

Posted on October 26, 2013 by admin

10:00a – 8:00p Banff report (working remotely) Trimming scientific progress section (whole report needs to fit in 4 pages) added more participant comments into all sections (I think this is more meaningful the organizer summaries). STORM4 Record chromatic bead data. … Continue reading →

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Friday 10/25/13

Posted on October 25, 2013 by admin

10:00a – 9:00p Ph Meeting (10:30-1:00p) More numbers simple fig for on rate / off rate model descrimination need more bio-info trends Psc knockdown Mentoring Discussing Random Walk simulation algorithms explaining existing model code presented stripped down model code for … Continue reading →

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Thursday 10/24/13

Posted on October 24, 2013 by admin

8:40a – 6:20p, 11:30p – 12:55a Goals Fix Ph model — neigbhors of neighbors in situs day 2 Cell Staining Hot washes (20 min, 2x 9 min in oven + 1 min switch) 1x SSCT cold wash PBT wash 45 … Continue reading →

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