Monthly Archives: September 2013

Monday 09/30/13

Posted on September 30, 2013 by admin

10:00a – 11:00a, 6:20p – 9:50p Oligo cleanup and concentration measurements for short-RNAs (pure and raw) (1) E02 13 kb green (between R/Y) (2) E06 Epc (12 kb YELLOW) (3) E07 Tou (15 kb YELLOW) (4) E09 RED 8 kb … Continue reading →

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Protected: Coordinating chromatin STORM research goals

Posted on September 27, 2013 by admin

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Friday 09/27/13

Posted on September 27, 2013 by admin

9:00a – 4:30p Multiday Goals Write letter to Ting and company Design D mel. gene library complete ANT-C stains (G1-G5) (need G1 probe to complete). Probe Making PCR cleanups: Large Regions D11 100 kb percicentric Green D12 385 kb Yellow … Continue reading →

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Thursday 9/26/13

Posted on September 26, 2013 by admin

10:00a – 10:40p Goals Laundry email Ting & Co. draft letter on progress on goals on chromatin Analyze existing data for new spots: density, puncta etc. scale factors? (density vs. length or something?) Computer splitdax aborted in night when ProRAID … Continue reading →

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Wednesday 09/25/13

Posted on September 25, 2013 by admin

9:30a – 11:15p STORM stop O/N STORM start copying data to Monet from O/N start spot-finding analysis on yesterday’s O/N splitdax F6 data. G2 data also not analyzed: set up DaoSTORM analysis on Cajal G2 bead data looks sparse — … Continue reading →

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Tuesday 09/24/13

Posted on September 24, 2013 by admin

11:00a-4:30p, 8:00p-11:00p Goals: Cell culture plate cells on Poly-Lysine coverglass (with Bogdan ?) passage remaining cells (with Bogdan ?) Prep 9/2 plate and new plate for DNA FISH. Staining O/N primary staining of Pc and anti-flag. Probe making RNA cleanup … Continue reading →

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Monday 09/23/13

Posted on September 23, 2013 by admin

9:30a – 7:30p, 9:00p – 11:50p Meetings 10a, lab meeting 11:45a, meeting with Ting and Fred Need to send letter to team. Cell in situs Wash out primary probes STORM E6 (tertiary approach still) — very weak spots visible in … Continue reading →

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Protected: lab meeting 09/23/13

Posted on September 23, 2013 by admin

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Sunday 09/22/13

Posted on September 22, 2013 by admin

5:30p – 8:30p Bogdan / Rotation project prep: move black IVT reactions to top of upstairs -20C freezer new DNA FISH in situs E6 (E(Pc) region, 15kb) F6 (Y/R 140 kb) Stain E6/F6 originals with 5 uL + 1 uL … Continue reading →

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Notes for deep sequences of libraries

Posted on September 22, 2013 by admin

Bauer Core equipment training option 1 Amplify sublibraries * Buy NEB DNA kit, end repair * blunt end ligation alternative PCR primer gel-extraction Qubit concentration (nanodrop?) [optional] BioAnalyzer at Bauer Core. [optional] qPCR, dilute sample a few times. By Illumina … Continue reading →

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