Monthly Archives: April 2014

Wednesday 04/30/14

9:00a – 8:35p Project 2 see post. Chromatin project still imaging G08G06 sometime rather abruptly between 9a and 11a the 750 dyes completely stopped switching off. Microscope stuff Installing and aligning dual-view on STORM3 figured out how to exchange dichroic … Continue reading

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project 2 update: 04/30/14

rRNA continued Goals: do we have all the abundant rRNA transcripts annotated and assembled into our fasta file for screening? Annotate FPKMs into list of rRNA and tRNA more stupid issues not getting the right genes There are yeast genes … Continue reading

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Tuesday 04/29/14

9:30a – 10:00p Computer Cajal troubleshooting computer behaving very sluggish today, denying connections restarted Cajal. also removed all the memory DIMMs and reinstalled them. Cajal now recognizes 48 GB of RAM again (instead of just 24 GB). Project 2 moved … Continue reading

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Monday 04/28/14

9:00a – 4:50p, 9:30-9:55p STORM coding stuff Code Organization Moved WarpPoints.m and CorrAlign.m into ChromeWarps Commited GitScripts, matlab-functions and matlab-storm z-calibration issues Jeff reports substantial differences between beads and single-molecules on the 10-15% scale should still be valid for separating … Continue reading

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Sunday 04/27/14

Goals: Read and send comments on latest version of Ajaz’s manuscript project 2 update write to XZ about poster for HHMI meeting write to XZ about June application for K99 Progress Project 2: see post Finished reading commenting up through … Continue reading

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Protected: project 2 update 04/28/14

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Saturday 04/26/14

coding working on rewrite of chromewarp code: ComputeChromeWarp.m this is now a properly functioning and tested function. Needs to be integrated into STORMfinder. should operate with a simplier menu — would like to be able to specify a list of … Continue reading

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Wednesday 04/23/14

8:30a – 10:45p Career planning from Jeff: K99 next application deadline is June 12th. After that it’s October 12th (see NLM page, NIGMS not as clearly laid out). recommended to apply with time to submit resubmission ~8 months after original … Continue reading

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Protected: project 2 update: 04/23/14

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Tuesday 04/22/14

9:30a – 8:30p issues with z-calibration fixed bug missing sqrt() in wx = w./sqrt(ax) and wx = w./sqrt(ax) take zcal bead images. Run Z-calibration script on these to generate curve fits use the curve fits to refit the beads, plot … Continue reading

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