Monthly Archives: March 2014

Monday 03/31/14

9:45a – 12:25a Chromatin Project Microscopy sessions! STORM2 Lib2 mutlicolor imaging. make fresh MEA make fresh Glox check stains, find a good stain to image Wed or Thur night. if cells and spots are dense 10 regions can be a … Continue reading

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Protected: project 2 experiments 03/31/14

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Protected: work with Pallav

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Sunday 03/30/14

10:40a – 12:15a Some basic organization matlab-functions collaboration discussed plans make headers match more strictly move all user prompts to pop-up boxes not command line prompts Project organization folder structure change all scripts folders to short-cuts or symbolic links to … Continue reading

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Protected: thoughts on chromatin structure

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Saturday 03/29/14

10:15a – 12:15a Multi-color data analysis Analyzing chromatic bead data from 2014-02-27 and 02-28 Analyzing drift bead data from 2014-02-27 / 28 should write a little script to auto launch this stuff? 2014-02-28 F06F05 dataset, Image 25 and on, Conventional … Continue reading

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Friday 03/28/14

9:45a – 11:50p Goals Record bead fields. check isoforms differences and consequences for probes selected (do our probes still hit the most popular isoform of imr90s?) Cell Staining washout stains for new multi-color samples. Need to wait until oven is … Continue reading

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Thursday 04/27/14

10:15a – 12:30a Goals Data processing copy C05 and C06 data to hard-drives Launch analysis of C03-C06 data plate, fix, prep and hybe new cells Probe making RT reactions test gel probe purfication cell staining wash out probes image multi-color … Continue reading

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Wednesday 03/26/14

9:20a – 10:10p, remotely, 10:50p – 11:30p Goals passage cells washout probe from new multicolor stain finish updating notes from yesterday STORM run configurations data analysis launched break down PRISM2 run of L3C04. (complete) break down STORM2 run of L3C03 … Continue reading

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Tuesday 03/25/14

10:00a – 1:00a Oligo Secondaries Fixes for secondary figure use the dots only rendering method make dots only rendering method a standard plugin for matlab-storm (I rather like it) wrote function StormHist.m added to QuickPlots of matlab-functions instead, not sure … Continue reading

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