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Monthly Archives: September 2015
Friday 09/04/15
9:00 am – 8:00 pm Presentations rehearsing lab meeting presentation (9am-9:30am) start cufflinks running on newly aligned NextSeq data (9:30 am – 10:00 am) lab meeting, 10am – 12:30 pm playing with deep seq data (needed to rerun cufflinks, overwrote … Continue reading
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Thursday 09/03/15
9:00 am – 7:10 pm, 9:00 pm – 10:00 pm, Goals finish lab meeting presentation for tomorrow finish CONTE center presentation for tomorrow finish update slides for discussion tomorrow Analysis analyze WT ANTC-G6 data Morning tasks check STORM imaging movies … Continue reading
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Wednesday 09/02/15
10:00 am – 8:00 pm, 9:00 pm – 11:20 pm Ph KD BXC-F6 further imaging STORM sample chamber contaminated by Draq5 — can’t see dye switching CycA cytoplasmic antibody stain still present after hybridization Sample prep preparing new sample with … Continue reading
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Tuesday 09/01/15
9:30 am – 8:15 pm, 9:30 pm – 11:30 pm STORM continued imaging ANT-C G6 in WT cells (imaging finished at 6:17 pm) Staining rinsed new antibody stains (CycA and Ph) CycA (after formamide) now only stains the cytoplasmic fraction … Continue reading
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