Categories
- AP patterning (13)
- Blog (1)
- Chromatin (88)
- Conference Notes (72)
- Fly Work (54)
- General STORM (25)
- Genomics (134)
- Journal Club (22)
- Lab Meeting (66)
- Microscopy (79)
- Notes (1)
- probe and plasmid building (58)
- Project Meeting (3)
- Protocols (13)
- Research Planning (74)
- Seminars (21)
- Shadow Enhancers (59)
- snail patterning (40)
- Software Development (5)
- Summaries (1,412)
- Teaching (9)
- Transcription Modeling (40)
- Uncategorized (10)
- Web development (19)
Links
Tags
analysis cell culture cell labeling chromatin cloning coding communication confocal data analysis embryo collection embryo labeling figures fly work genomics hb image analysis image processing images in situs Library2 literature making antibodies matlab-storm meetings modeling MP12 mRNA counting Ph planning presentation probe making project 2 project2 result results sectioning section staining shadow enhancers sna snail staining STORM STORM analysis troubleshooting writing-
GitHub Projects
Monthly Archives: October 2015
Protected: lab meeting 10/30/15
There is no excerpt because this is a protected post.
Posted in Summaries
Comments Off on Protected: lab meeting 10/30/15
Friday 10/30/15
9:30 am – 5:00 pm Applications working on Harvard MCB application applications won’t submit requests to letter writers until application is complete. Princeton application also won’t submit requests to letter writers given that a motivated candidate would like to have … Continue reading
Posted in Summaries
Comments Off on Friday 10/30/15
Tuesday 10/27/15
9:00 am Deep seq final prep running tape-station samplesname, conc pg/uL, index, tube Ph1, 496, 1, A M1, 442, 2, A M2, 665, 4, – 10-22 #1, 652, 1, B 10-22 #2, 516, 2, B 2W, 617, 3, B 2P, … Continue reading
Posted in Summaries
Comments Off on Tuesday 10/27/15
Monday, 10/26/15
9:30 am – 7:00 pm, 9:00 pm – 11:00 pm Deep seq prep continued with deep-seq prep finished second strand synthesis ran clean up on DNA-XP beads from Bauer ran adaptor ligation. premixed adaptor and enzyme mix (2 min on … Continue reading
Posted in Summaries
Comments Off on Monday, 10/26/15
Sunday 10/25/15
8:30 pm – 11:15 pm RNA deep-seq prep sample order (8 samples): 10-22 n1, 10-22 n2, 2W, 2P, 2-4W, 2-4P, 4W, 4P running sample isolation by magnetic polyT beads, following manufacturer’s protocol. 3 samples dehydrated in last elution step. I … Continue reading
Posted in Summaries
Comments Off on Sunday 10/25/15
Friday 10/23/15
9:00 am pairing meeting rehearse talk (8:45-9:15) see notes Experiments: QPCR for RNAi (9:15 am – 11:05 am) RNA concentrations from yesterday: 600 – 1200 ng/uL (55 uL volume) ran RNase H digestion last night, at 4C in block O/N. … Continue reading
Posted in Summaries
Comments Off on Friday 10/23/15
Thursday, 10/22/15
9:15 am – 6:30 pm presentations revising presentation for Pairing meeting with suggestions from BB (9:30 am – 12:30 pm) practice presentation Experiments 1 well of Ph KD and WT took through the RNAi treatment / wash / serum starve … Continue reading
Posted in Summaries
Comments Off on Thursday, 10/22/15
Wednesday 10/21/15
8:30 am – 7:00 pm background reading background research on subtelomeres for new XZ (8:30 a – 10:00 a) see notes and email. presentation prep working on polycomb bridging presentation for pairing meeting (2:30 – 7:00 pm) Imaging (10:00 am-1:00 … Continue reading
Posted in Summaries
Comments Off on Wednesday 10/21/15
Tuesday 10/20/15
10:15 am – 7:30 pm Goals Finish RNA hybes and check hb RNA stains Finish embryo BX-C 15 kb region hybes and check stains Finish draft of ppt presentation for pairing meeting. Probe synthesis Mix: 20 uL RNA 8 uL … Continue reading
Posted in Summaries
Comments Off on Tuesday 10/20/15
Monday 10/19/15
10:00 am – 6:50 pm Tasks send info to Stanford Dev Bio. finish and submit application to Cornell Physics (due today) write to XZ about new experimental design. synthesize lots more dsRNA start new KD of Ph experiment with 2 … Continue reading
Posted in Summaries
Comments Off on Monday 10/19/15