Monthly Archives: October 2015

Protected: lab meeting 10/30/15

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Friday 10/30/15

9:30 am – 5:00 pm Applications working on Harvard MCB application applications won’t submit requests to letter writers until application is complete. Princeton application also won’t submit requests to letter writers given that a motivated candidate would like to have … Continue reading

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Tuesday 10/27/15

9:00 am Deep seq final prep running tape-station samplesname, conc pg/uL, index, tube Ph1, 496, 1, A M1, 442, 2, A M2, 665, 4, – 10-22 #1, 652, 1, B 10-22 #2, 516, 2, B 2W, 617, 3, B 2P, … Continue reading

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Monday, 10/26/15

9:30 am – 7:00 pm, 9:00 pm – 11:00 pm Deep seq prep continued with deep-seq prep finished second strand synthesis ran clean up on DNA-XP beads from Bauer ran adaptor ligation. premixed adaptor and enzyme mix (2 min on … Continue reading

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Sunday 10/25/15

8:30 pm – 11:15 pm RNA deep-seq prep sample order (8 samples): 10-22 n1, 10-22 n2, 2W, 2P, 2-4W, 2-4P, 4W, 4P running sample isolation by magnetic polyT beads, following manufacturer’s protocol. 3 samples dehydrated in last elution step. I … Continue reading

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Friday 10/23/15

9:00 am pairing meeting rehearse talk (8:45-9:15) see notes Experiments: QPCR for RNAi (9:15 am – 11:05 am) RNA concentrations from yesterday: 600 – 1200 ng/uL (55 uL volume) ran RNase H digestion last night, at 4C in block O/N. … Continue reading

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Thursday, 10/22/15

9:15 am – 6:30 pm presentations revising presentation for Pairing meeting with suggestions from BB (9:30 am – 12:30 pm) practice presentation Experiments 1 well of Ph KD and WT took through the RNAi treatment / wash / serum starve … Continue reading

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Wednesday 10/21/15

8:30 am – 7:00 pm background reading background research on subtelomeres for new XZ (8:30 a – 10:00 a) see notes and email. presentation prep working on polycomb bridging presentation for pairing meeting (2:30 – 7:00 pm) Imaging (10:00 am-1:00 … Continue reading

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Tuesday 10/20/15

10:15 am – 7:30 pm Goals Finish RNA hybes and check hb RNA stains Finish embryo BX-C 15 kb region hybes and check stains Finish draft of ppt presentation for pairing meeting. Probe synthesis Mix: 20 uL RNA 8 uL … Continue reading

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Monday 10/19/15

10:00 am – 6:50 pm Tasks send info to Stanford Dev Bio. finish and submit application to Cornell Physics (due today) write to XZ about new experimental design. synthesize lots more dsRNA start new KD of Ph experiment with 2 … Continue reading

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