10:30 A – 11:15 P
- fix MP08 and wt embryos, 1.5-4 hrs
- MP09 embryos now in resin embedding O/N
- new antibody stains (bcd, hb; ftz, nuclear lamin-B, H3K4me3, H3K27me3) in 15 min F fixed embryos. 2 hr primary at RT, then O/N primary at 4C.
- O/N confocal of new MP08 embryos — several very good samples. Expression levels generally quite lower. Found one good completely-gastrulated. Not imaging. LacZ / esg combo works well as JB promised.
- working on layout of snail dynamics figure.