9:40A – 11:55 P
- Lab meeting presentation: Jeff Moffit
- 1 hr RT O/N primaries
- washing out O/N primary antibody labels
- ACS draft app to Matt
- Fly meeting receipts organized, sent to Matt
- Promoter synchrony paper feedback for Mounia and Jacques
- KE run gel of PCR amplified templates. Finish extraction tomorrow.
- ran out of mounting medium
- ubx looks great in short fix, eve is detectable, Ftz looks better in heat-fix (probably better still in short fix), Bcd looks better in short fix than heat-fix.
- Confocal repeatedly dramatically changes z-position — record position and return to it minutes later and the same original plane is hundreds of microns away from where recorded. Very frustrating.
- Troubleshooting issues with batch GUI code and drift correction — tried splitting up image into different temporal segments to do drift correction. All outputs are the same. This wasn’t happening earlier when it was just integrating sub-images rather than splitting them up.