Wednesday 07/11/12

10:10 A – 5:ooP

  • check antibody locations with KE
  • in situs day 3
  • Confocal: tub and Ant-C genes look good.  Preliminary data suggests loci mostly paired in older embryos but not condensed — substantial separation distance.
  • Ubx and AbdB have good expression overlap.  Preliminary data suggests quite close (not entirely a posterior first repression then?)
  • add McGinnis CB data to Fig 2 heterogeneity
  • working on cover letter for snail kinetics paper
  • post-fixed in situs, some embryos set aside in PBT for mounting (ProLong gold missing?)
  • embedding in situs.  Forgot tertiary antibodies (H3-750) :(.
  • Flipped fly stocks.
  • 3x Mat Gal4 stuggling badly.  Down to 2 new bottles.
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