Wednesday 09/12/12

9:20A – 7:00P

  • STORM4 status
    • Laser powers: 488 68mW, 561 50mW, 656 78mW.
    • Neither table is floating
    • At first system failed to connect properly to AOTF
    • Fixed AOTF by reboot, but system now fails to connect to focus-lock IR camera.
    • Shut everything down, letting cool, attempt clean reboot. 
    • STORM4 working.  Beam size much too small with 60 mm lens.  Swapped out for 150mm lens (treat fiber end as a point source). 
  • Etched 20 + 40 min E(Pc) slide
  • STORM imaging E(Pc) slide
    • Bead images with 1:25,000 bead dilution bead slide (tired of occassional overlapping beads screwing things up)
    • New beadmap region file, same number of images, scans 5x larger area.  
  • Inspected thin sections of oligo-STORM: no staining evident. 🙁
  • Monet restarted without permission O/N.  

  • Cajal doesn’t seem to understand ‘exit’ command at end of insight: gives system errors program crashed.
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