Laser powers
- 750 at 1700 mA is 209 mW at meter
- 647 at 200 mW source is 120 at meter. 300 mW source is 190 at meter
- 488 at 75% power is 200 mW at meter
- 405 at max is 2.9 mW
Bead imaging
Cell imaging
- H3K9me2 750 much brighter in IF first sample. Also switching much longer.
- 647 DFISH much brighter in DFISH first sample.
- substantial drift during several videos (e.g. 647-H3K9cent_0001). Hopefully drift correction calculations clean this up.