z-calibration: Insight vs DaoSTORM

with single iteration, Hazen 3D-DaoSTORM avoids splitting molecules more distant from frame

A few notes on 3D-DaoSTORM

• First 4 iterations are computed with 4x the input threshold. So if using less than 4 iterations you want to quarter the threshold to get the same results.
• 3D-DaoSTORM iterative subtracts the fitted pointspread function (with whatever elliptical fit it computed).
• 3D-DaoSTORM produces separate ‘averaged-molecule lists’, *_alist.bin and raw molecule lists *_mlist.bin

same calibration movies for all 4 channels computed from DaoSTORM and Insight

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fig_750_zcal_0001_zcal_curves

fig_dao_750_zcal_0001_zcal_curves

fig_dao_488_zcal_0001_zcal_curves

fig_488_zcal_0001_zcal_curves

fig_561_zcal_0001_zcal_curves

fig_dao_561_zcal_0001_zcal_curves

fig_dao_647_zcal_0001_zcal_curves

fig_647_zcal_0001_zcal_curves