Wednesday 05/15/13

Posted on May 15, 2013 by admin

10:15 A – 7:30P, 8:30P – 12:30 A

Probe design

  • ordered new 405 oligo-probes with short attachment
  • ordered unlabeled 30 bp AATAT probes with primary extentions
  • ordered unlabeled 30 bp AATAT probes with double length primary extention to bind 32mer 405 and cy5
  • ordered 32mer NHS-ester and cy5

Clone 8 cluster analysis

  • substantially more disperse? or is this background?
  • compare S2 and clone8
    1. BX-C transcriptomics gene expression
    2. Pol II density
    3. Histone1 density and Histone3 density
    4. All tracks look very similar. Probably not different structure.
  • line is derived from L3 wing disks.
  • Definetely need in-image feducials.

STORM

  • Make PDMS box with Jeff to allow for repeated stain/image/stain cycles.
  • Trying to STORM embyro-AATAT cy5 with beads. 540/560 and 580/065 beads really only bright enough with 561 laser on, not excited by 647 laser.
  • 580 beads washed off or bleached in STORM buffer
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