10:15a – 8:10p, 8:50p-11:05p
BX-C tiling
- Finish O/N STORM of Ubx spots
- start 65C 50% formamide rinse out of probe (30 min)
- Hot wash of newly labeled Ubx (orginally AbdA): 60C 2x SSCT
- Image Ubx. Correspondence of cells and correspondence of dots looks good. Hatching + steve works.
- Zero feducial beads remain attached for all 3 cells :(.
- Try crosslinking beads.
STORM analysis
- copy BX-C tiling data to ProRAID
- Need to develop better pipeline for splitting and analyzing dax.
- Successfully tested stub-function.
Ph data analysis
- Manually crop nuclei from images for batch processing with new analysis script
- Wt-Ph-flag ims 0-2 not fully drift corrected, spreads out Pc into non-clusters.
- Reading and writing to data-drive is pretty in-efficient.
- im4-5 (and on) look much better (drift stabilized? better illumination?)
- have to be careful on non-expressing cells (especially in PhM).
- sent update to Ajaz.
