Tag Archives: coding

Wednesday 01/16/13

Posted on January 16, 2013 by admin

9:30 A – 7:30 P  Finish comments on counting comparison for ML # Finish revisions Write to MERCK about Probe Making Precipitate and resuspend RNA-FISH DNP probes for tou, inv, en, Antp and Dfd. Make new RNA-Hybe soln to resuspend … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , , | Comments Off on Wednesday 01/16/13

Tuesday 01/15/13

Posted on January 15, 2013 by admin

10:00 A – 7:00P, 8:30P-11:30P Probe making in vitro transcription note, spreadsheet is interactive, all reactant amounts aside from template DNA (depends on spec’d conc) computed by sheet. Coding working on new code (STORMfinder) to replace interactive selection of dot … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Tuesday 01/15/13

Sunday 12/09/12

Posted on December 9, 2012 by admin

10:20 A – 7:30P, 9:30-11:00P STORM 750 1600 mA = 18 mW 1.45 mW 405 200 mW set 647 Made new Glox 750 not switching well.  Not sure if it’s just low labeling (405 looked okay but not strong). 647 … Continue reading →

Posted in Summaries | Tagged , , , , , | Comments Off on Sunday 12/09/12

Saturday 11/17/12

Posted on November 17, 2012 by admin

10:00 A – 12:00P, 3:30P – 8:00P, 9:00P-11:20P S2 Cell cent + chromatin/PcG Hot washes PBT washes block 4 hrs antibodies: H3K9me2, Pc, PCNA (Fisher) (1:3 across the top) rinse, block, secondaries O/N at 4C Harvard wins Yale Game Chromatic … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Saturday 11/17/12

Matlab Coding guidelines

Posted on October 14, 2012 by admin

  On writing readable matlab code [In development] Rule 1: The code you run and re-run on different stuff should be functions Functions can be shared / read used by others.  Scripts generally cannot.  Script with front-matter inputs = bad … Continue reading →

Posted in Research Planning | Tagged | Comments Off on Matlab Coding guidelines

Saturday 10/13/12

Posted on October 13, 2012 by admin

11:00A – 6:ooP, 9:ooP-11:00P binomial statistics and confidence intervals discussion with JM Collect virgin E(spl) and sim[D] flies, 4 more vials of crosses. Finished virgin collections and cross-setups for present.  All flies now on RT shelf.  Organizing Matlab STORM library … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Saturday 10/13/12

Thursday 10/11/12

Posted on October 11, 2012 by admin

9:30 AM – 7:45 P, 9:00P – 11:15 PM Fly Work Collect virgin MTDs, E(spl) and sim[D] Collect male E(spl), sim[D] (none) and Scm.  Su(Z)12 stock struggling, would be good choice for knockdown. Set up MTD x Psc (and MTD … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Thursday 10/11/12

Tuesday 10/09/12

Posted on October 9, 2012 by admin

10:00 A – 7:20P, 8:30P – 11:30P Fly Work collect virgin MTDs.  (progeny of MTD x TRIP -> collection cage.   These embryos should lay infertile offspring / transformed embryos).  Let’s do Psc; MTD first, see if structure of K27me3 … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Tuesday 10/09/12

Monday 10/08/12

Posted on October 8, 2012 by admin

10:45 A – 6:00P, 8:00P-9:00P Fly Work Flip fly stocks clear MGD bottles for virgin collection clear sim[D] and Espl[D] for virgin collection. start expanding double-balancer stock collect virgin MGD flies Coding remove save data troubleshooting scripts from STORMrender (dramatically … Continue reading →

Posted in Fly Work, Summaries | Tagged , | Comments Off on Monday 10/08/12

Sunday 10/07/12

Posted on October 7, 2012 by admin

8:30 AM -5:30PM Development of Matlab GUI based STORM suite converting data analysis portions of movie2vector script into GUI STORManalyzer functionalizing substeps in new function format. fixed bug in modify_script.m functional STORManalyzer clones all functions of movie2vectorSTORM up

Posted in Summaries | Tagged | Comments Off on Sunday 10/07/12