Tag Archives: confocal

Thursday 03/22/12

Posted on March 22, 2012 by admin

9:45 A -12:20 A O/N confocal run failed horribly.  Despite warm-up, quick reset of positions, and quick calibration run, devastating z change of focus occurred during run. Rinsing out primary antibodies GPU multi-fit code still running slow (70 min+ per channel per … Continue reading →

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Wednesday 03/21/12

Posted on March 21, 2012 by admin

12:00P – 9:30P (9A-12P lab volleyball practice) in situs day 2 protein stains wash out primaries, post-fix, add secondaries. started new page, see record: Kill old bottles of MP09, yw.  Need to set up new bottles STORM imaging of 3Mb … Continue reading →

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Sunday 03/18/12

Posted on March 18, 2012 by admin

11:00 A – 8:45 P No colonies on plates Finish O/N STORM run.  Run failed at movie 121, unknown error message. attempt bead feducial drift correction: polysterene beads good autofluoresnce in all but 750, but bleach fast. 405 beads not … Continue reading →

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Thursday 03/15/12

Posted on March 15, 2012 by admin

9:45 A – 9:30P minor z drift issues still observed in STORM run. Time to take vertical beads for z-fit.  at 1:1000 density is a bit low. try 1:500 using ND 2-3 for z-fit bead images. Might be saturated?  Need … Continue reading →

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Protected: Super-resolution imaging of PcG bodies

Posted on February 27, 2012 by admin

There is no excerpt because this is a protected post.

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Saturday 02-25-12

Posted on February 25, 2012 by admin

10:45 A — 6:00P, 8:00P — 12:45 A in situs day 3 working on 3D data analysis Calibrated 750 channel from bead images Confocal: image S2 330 nm (looks like intronic en stain and mature mRNA detectable). Confocal: image Psc-hox … Continue reading →

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Thursday 02/23/12

Posted on February 23, 2012 by admin

10:05 A – 12:10A PcG and Bcd staining embryos incubating in primary at RT for final hour. Flip fly plates Discussion with John about projects Need to look up Drosophila repeat sequences for probe testing. in situs day 1 on … Continue reading →

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Wednesday 02/22/12

Posted on February 22, 2012 by admin

9:30 A – 8:50 PM Sectioning: 300 nm. trapazoids work better, long edge at bottom. Cut 3 coverslips of S6.  Two at 330 nm, one at 100 nm. Coverslip 1 at 330 nm is tiled from upper left to lower … Continue reading →

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confocal test summary

Posted on February 22, 2012 by admin

confocal of:  

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Saturday 02/19/2012

Posted on February 20, 2012 by admin

10:00A – 5:00P washing out final antibody from in situs. embryos moved to ethanol WGA staining Hox in situs 1:10 set up to try cutting thicker samples.  Using Yari’s new walled area for microtome. select Images for Cell feature on … Continue reading →

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